Anti-GNAI2 抗体 [EPR9469] (ab157204)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9469] to GNAI2
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-GNAI2 antibody [EPR9469]
GNAI2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9469] to GNAI2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human GNAI2. The exact sequence is proprietary.
Database link: P04899 -
ポジティブ・コントロール
- Recombinant Human GNAI2 (ab134531) can be used as a positive control in WB. U937 and U-87 MG cell lysates; fetal brain lysate; kidney and pancreas tissues; U937 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9469 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157204の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 40 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. -
配列類似性
Belongs to the G-alpha family. G(i/o/t/z) subfamily. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cell membrane. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody. - Information by UniProt
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参照データベース
- Entrez Gene: 2771 Human
- Entrez Gene: 14678 Mouse
- Entrez Gene: 81664 Rat
- Omim: 139360 Human
- SwissProt: P04899 Human
- SwissProt: P08752 Mouse
- SwissProt: P04897 Rat
- Unigene: 77269 Human
see all -
別名
- Adenylate cyclase inhibiting G alpha protein antibody
- Adenylate cyclase-inhibiting G alpha protein antibody
- GIP antibody
see all
画像
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All lanes : Anti-GNAI2 antibody [EPR9469] (ab157204) at 1/10000 dilution
Lane 1 : fetal brain lysate
Lane 2 : U937 cell lysate
Lane 3 : U-87 MG cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
ab157204 showing +ve staining in Human normal liver.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab157204 showing +ve staining in Human normal brain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling GNAI2 using ab157204 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GNAI2 using ab157204 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of permeabilized U937 cells labeling GNAI2 (red), using ab157204 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab157204 は 8 報の論文で使用されています。
- Fan Y et al. Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons. Commun Biol 5:411 (2022). PubMed: 35505181
- Mechin V et al. Histological and Immunohistochemical Characterization of Vomeronasal Organ Aging in Mice. Animals (Basel) 11:N/A (2021). PubMed: 33922332
- Chen X et al. Translational control by DHX36 binding to 5'UTR G-quadruplex is essential for muscle stem-cell regenerative functions. Nat Commun 12:5043 (2021). PubMed: 34413292
- Haase J et al. Sex and brain region-specific regulation of serotonin transporter activity in synaptosomes in guanine nucleotide-binding protein G(q) alpha knockout mice. J Neurochem 159:156-171 (2021). PubMed: 34309872
- Caggia S et al. Small Molecule Inhibitors Targeting Gai2 Protein Attenuate Migration of Cancer Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32575572
- Lu Y et al. SNHG1 Inhibits ox-LDL-Induced Inflammatory Response and Apoptosis of HUVECs via Up-Regulating GNAI2 and PCBP1. Front Pharmacol 11:703 (2020). PubMed: 32536864
- Touhara KK & MacKinnon R Molecular basis of signaling specificity between GIRK channels and GPCRs. Elife 7:N/A (2018). PubMed: 30526853
- Bissinger R et al. Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gai2. Sci Rep 6:30925 (2016). WB . PubMed: 27499046