Anti-G-CSF 抗体 (ab9691)

Reviews (6)Q&A (8)References (7)

Key features and details

  • Rabbit polyclonal to G-CSF
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Human
  • Isotype: IgG

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5D7] to G-CSF (ab9818).

This antigen affinity purified antibody, in conjunction with ab271256 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF

ターゲット情報

  • 機能

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

  • 配列類似性

    Belongs to the IL-6 superfamily.

  • 翻訳後修飾

    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C17orf33 antibody
      • Colony stimulating factor 3 (granulocyte) antibody
      • Colony stimulating factor 3 antibody
      • CSF 3 antibody
      • CSF beta antibody
      • CSF3 antibody
      • CSF3_HUMAN antibody
      • CSF3OS antibody
      • Csfg antibody
      • Filgrastim antibody
      • G-CSF antibody
      • GCSA antibody
      • GCSF antibody
      • Granulocyte colony stimulating factor antibody
      • Granulocyte colony-stimulating factor antibody
      • Lenograstim antibody
      • Macrophage granulocyte inducer 2 antibody
      • MGC45931 antibody
      • MGI 2 antibody
      • Pluripoietin antibody
      see all

    画像

    • Western blot - Anti-G-CSF antibody (ab9691)
      Western blot - Anti-G-CSF antibody (ab9691)
      Anti-G-CSF antibody (ab9691) at 1 µg/ml + Recombinant human G-CSF protein (ab73371) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 2 minutes
    • Sandwich ELISA - Anti-G-CSF antibody (ab9691)
      Sandwich ELISA - Anti-G-CSF antibody (ab9691)
      Standard Curve for G-CSF (Analyte: ab51298) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [5D7] to G-CSF (ab9818) at 1ug/ml and Detector Antibody Rabbit polyclonal to G-CSF (ab9691) at 0.5ug/ml

    参考文献 (7)

    ab9691 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9691 は 7 報の論文で使用されています。

    • Andar A  et al. Wood Microfluidics. Anal Chem 91:11004-11012 (2019). PubMed: 31361950
    • Guo L  et al. A high-risk luminal A dominant breast cancer subtype with increased mobility. Breast Cancer Res Treat N/A:N/A (2019). PubMed: 30778902
    • Meyer MA  et al. Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance. Nat Commun 9:1250 (2018). PubMed: 29593283
    • Zhang L  et al. G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma. Cancer Manag Res 10:2767-2775 (2018). PubMed: 30147373
    • Makino T  et al. Diffuse fluorodeoxyglucose-positron uptake in the bone marrow of a patient with granulocyte colony-stimulating factor-producing pleomorphic carcinoma of the lung: A case report. Mol Clin Oncol 7:103-106 (2017). PubMed: 28685085
    • Xue H  et al. Tiaogeng Yijing decoction improves the pregnancy outcomes of patients with poor ovarian response undergoingin vitrofertilization-embryo transfer. Exp Ther Med 14:3935-3941 (2017). PubMed: 29043003
    • Hollmén M  et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). IHC-P ; Human . PubMed: 27141367

    レビューと Q&A

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    1-10 of 14 Abreviews or Q&A

    Question

    Hi can you please specify for our records what would be the expiry date after reconstitution of the lyophilized material as suggested by you.

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    Answer

    The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8oC. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
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    Abcam user community

    Verified customer

    投稿 May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    投稿 May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% Formalin
    Read More

    Abcam user community

    Verified customer

    投稿 May 19 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-G-CSF antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% Normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    投稿 May 19 2017

    Western blot abreview for Anti-G-CSF antibody

     Inconclusive
    Abreviews
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Rat Cell lysate - whole cell (PC-12 cells)
    Specification
    PC-12 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Abcam user community

    Verified customer

    投稿 Jun 17 2013

    Question

    For product ab9691 (anti-G-CSF antibody), are there recommended expiration/stability dates for the lyophilized and the reconstituted product, stored at -20°C?

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    Answer



    There is no real expiry date on all our antibodies. Usually the life is 10 years if stored in -20. Minimal freeze thaw cycles definitely extend the life of the antibody.

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    Question

    Dear order team,

    Our customer would like to purchaseab9691 with Lot#GR7283-8.
    Please let me know if you havesame lot#in stock.
    Thank you in advance.

    Best regards,

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    Answer

    Thank you for contacting us. We do not currently have that particular lot in stock but we do have lots of antibody produced from the same bleed and purification. The only difference would be that the antibody was aliquotted by us at a different time. The lots available are GR7283-9 and GR7283-10. Please specify when placing the order that you would like one of these.

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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    Question

    How long can this antibody be stored at -20C?

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    Answer

    Thank you for your call today and for your question.

    We don't assign specific expiration dates for our products (our guarantee is for 6 months after purchase), but we expect an antibody stored at -20C to be viable for up to 5 years.

    I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you. Have a nice day!

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    Question

    Dear Technical, Good day! We have a customer who has purchased the above antibody and would like a certificate of analysis for this.

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    Answer

    Thank you for contacting. Please find attached the Certificate of Analysis for ab9691. If there is anything else I can help you with, please let me know.

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    1-10 of 14 Abreviews or Q&A

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