Anti-Fyn 抗体 [FYN-01] (ab1881)

Reviews (3)Q&A (2)References (10)

Key features and details

  • Mouse monoclonal [FYN-01] to Fyn
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-Fyn antibody [FYN-01]
    Fyn 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [FYN-01] to Fyn

  • 由来種

    Mouse

  • 特異性

    The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases.

  • アプリケーション

    適用あり: Flow Cyt, ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment, corresponding to amino acids 7-176 of Human Fyn.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 59 kDa.

ターゲット情報

  • 機能

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.

  • 組織特異性

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • 細胞内局在

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

    • 別名

      • C syn protooncogene antibody
      • Fyn antibody
      • FYN oncogene related to SRC FGR YES antibody
      • FYN_HUMAN antibody
      • OKT3 induced calcium influx regulator antibody
      • P59 FYN antibody
      • p59-Fyn antibody
      • Protein tyrosine kinase fyn antibody
      • Proto oncogene tyrosine protein kinase fyn antibody
      • Proto-oncogene c-Fyn antibody
      • Proto-oncogene Syn antibody
      • Protooncogene Syn antibody
      • SLK antibody
      • Src like kinase antibody
      • Src yes related novel gene antibody
      • Src-like kinase antibody
      • Src/yes related novel antibody
      • SYN antibody
      • Tyrosine kinase p59fyn T antibody
      • Tyrosine kinase p59fyn(T) antibody
      • Tyrosine-protein kinase Fyn antibody
      see all

    画像

    • Western blot - Anti-Fyn antibody [FYN-01] (ab1881)
      Western blot - Anti-Fyn antibody [FYN-01] (ab1881)
      Anti-Fyn antibody [FYN-01] (ab1881) at 1 µg/ml + Recombinant human Fyn protein (ab84696) at 0.01 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 59 kDa


      Exposure time: 10 seconds
    • Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [FYN-01] (ab1881)
      Immunocytochemistry/ Immunofluorescence - Anti-Fyn antibody [FYN-01] (ab1881)
      Immunocytochemistry/ Immunofluorescence analysis of human primary fibroblasts labelling Fyn with ab1881 at 5 µL. Actin cytoskeleton was stained with phalloidin (red) and cell nuclei were stained with DAPI (blue). 
    • Flow Cytometry - Anti-Fyn antibody [FYN-01] (ab1881)
      Flow Cytometry - Anti-Fyn antibody [FYN-01] (ab1881)

      Overlay histogram showing Ramos cells stained with ab1881 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    参考文献 (10)

    ab1881 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1881 は 10 報の論文で使用されています。

    • Yu L  et al. c-Maf inducing protein inhibits cofilin-1 activity and alters podocyte cytoskeleton organization. Mol Med Rep 16:4955-4963 (2017). PubMed: 28791377
    • Bouchet J  et al. Rab11-FIP3 Regulation of Lck Endosomal Traffic Controls TCR Signal Transduction. J Immunol 198:2967-2978 (2017). PubMed: 28235866
    • Suo M  et al. Role of Fyn-mediated NMDA receptor function in prediabetic neuropathy in mice. J Neurophysiol 116:448-55 (2016). PubMed: 27146985
    • Higa-Nakamine S  et al. Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone. J Biol Chem 290:25974-85 (2015). PubMed: 26338704
    • McGinnis LK & Kinsey WH Role of focal adhesion kinase in oocyte-follicle communication. Mol Reprod Dev 82:90-102 (2015). PubMed: 25536210
    • Monin A  et al. Glutathione deficit impairs myelin maturation: relevance for white matter integrity in schizophrenia patients. Mol Psychiatry N/A:N/A (2014). Human . PubMed: 25155877
    • Takai S  et al. Distinct effects of saracatinib on memory CD8+ T cell differentiation. J Immunol 188:4323-33 (2012). IP . PubMed: 22450814
    • Randriamboavonjy V  et al. AMPK {alpha}2 subunit is involved in platelet signaling, clot retraction, and thrombus stability. Blood 116:2134-40 (2010). WB ; Mouse . PubMed: 20558612
    • Mitchell LA  et al. Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa. Mol Hum Reprod 14:235-43 (2008). PubMed: 18245108
    • Hauri-Hohl MM  et al. Donor T-cell alloreactivity against host thymic epithelium limits T-cell development after bone marrow transplantation. Blood 109:4080-8 (2007). PubMed: 17213290

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    Question

    Inquiry: I would like to buy an anti-Fyn antibody made in mouse that detects fyn form human and mouse tissues. This product 1881 says it is like this but the picture of the blot shows a band higher than 59 KDa and also says it was used an anti-goat secondary antibody. could you clarify about all this?

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    Answer

    Thank you for your inquiry.
    The image on the datasheet of ab1881 shows a WB with the positive control ab84696.
    ab84696 is a Active human Fyn full length protein that has a tag. The tag has a size of ca. 26kDa.
    Therefore the band at ca 85 kDa is what we would expect.
    If you are using a sample with endogenous Fyn the expected size is ca 59 kDa.
    I hope this information is helpful and wish you good luck with your research.

    Read More

    Immunocytochemistry abreview for Anti-Fyn antibody [FYN-01]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 15 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Fyn antibody [FYN-01]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Huh7 cells)
    Permeabilization
    Yes - Triton X-100
    Specification
    Huh7 cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 29 2020

    Western blot abreview for Anti-Fyn antibody [FYN-01]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Huh7 (liver cell9)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    200000 cells
    Specification
    Huh7 (liver cell9
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 25 2019

    Question

    I have received a product recall notification on ab15249.

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

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