Anti-Fyn 抗体 [FYN-01] (ab1881)
![Western blot - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.co.jp/ps/products/1/ab1881/Images/Fyn-Primary-antibodies-ab1881-2.jpg "Western blot - Anti-Fyn antibody [FYN-01] (ab1881)")
Key features and details
- Mouse monoclonal [FYN-01] to Fyn
- Suitable for: ICC/IF, ICC, IP, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Fyn antibody [FYN-01]
Fyn 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [FYN-01] to Fyn -
由来種
Mouse -
特異性
The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases. -
アプリケーション
適用あり: ICC/IF, ICC, IP, Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment, corresponding to amino acids 7-176 of Human Fyn.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
FYN-01 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1881の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| ICC | (1) |
Use a concentration of 5 µg/ml.
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| IP |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 59 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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ICC
Use a concentration of 5 µg/ml. |
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IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 59 kDa. |
ターゲット情報
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機能
Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3. -
組織特異性
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
細胞内局在
Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes. - Information by UniProt
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参照データベース
- Entrez Gene: 2534 Human
- Entrez Gene: 14360 Mouse
- Omim: 137025 Human
- SwissProt: P06241 Human
- SwissProt: P39688 Mouse
- Unigene: 390567 Human
- Unigene: 4848 Mouse
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製品の状態
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
別名
- C syn protooncogene antibody
- Fyn antibody
- FYN oncogene related to SRC FGR YES antibody
see all
画像
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Western blot - Anti-Fyn antibody [FYN-01] (ab1881)Anti-Fyn antibody [FYN-01] (ab1881) at 1 µg/ml +
Recombinant human Fyn protein (ab84696) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Exposure time: 10 seconds -
![Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.co.jp/ps/products/1/ab1881/Images/ab1881-455393-AntiFyn-antibody-Immunocytochemsitry.jpg)
Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)Immunocytochemistry analysis of Fyn in murine transformed fibroblasts using ab1881 (red) at 5 µL. Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
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![Flow Cytometry - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.co.jp/ps/products/1/ab1881/Images/Fyn-Primary-antibodies-ab1881-3.jpg)
Flow Cytometry - Anti-Fyn antibody [FYN-01] (ab1881)Overlay histogram showing Ramos cells stained with ab1881 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.co.jp/ps/products/1/ab1881/Images/ab1881-265226-anti-fyn-antibody-fyn-01-immunofluorescence.jpg)
Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)Immunofluorescence analysis of human primary fibroblasts labelling Fyn with ab1881 at 5 µL. Actin cytoskeleton was stained with phalloidin (red) and cell nuclei were stained with DAPI (blue). -
![Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)](https://www.abcam.co.jp/ps/products/1/ab1881/Images/ab1881-455392-AntiFyn-antibody-Immunocytochemsitry.jpg)
Immunocytochemistry - Anti-Fyn antibody [FYN-01] (ab1881)Immunocytochemistry analysis of Fyn in human HeLa cell line using ab1881 (green) at 5 µL. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab1881 は 10 報の論文で使用されています。
- Yu L et al. c-Maf inducing protein inhibits cofilin-1 activity and alters podocyte cytoskeleton organization. Mol Med Rep 16:4955-4963 (2017). PubMed: 28791377
- Bouchet J et al. Rab11-FIP3 Regulation of Lck Endosomal Traffic Controls TCR Signal Transduction. J Immunol 198:2967-2978 (2017). PubMed: 28235866
- Suo M et al. Role of Fyn-mediated NMDA receptor function in prediabetic neuropathy in mice. J Neurophysiol 116:448-55 (2016). PubMed: 27146985
- Higa-Nakamine S et al. Involvement of Protein Kinase D1 in Signal Transduction from the Protein Kinase C Pathway to the Tyrosine Kinase Pathway in Response to Gonadotropin-releasing Hormone. J Biol Chem 290:25974-85 (2015). PubMed: 26338704
- McGinnis LK & Kinsey WH Role of focal adhesion kinase in oocyte-follicle communication. Mol Reprod Dev 82:90-102 (2015). PubMed: 25536210
- Monin A et al. Glutathione deficit impairs myelin maturation: relevance for white matter integrity in schizophrenia patients. Mol Psychiatry N/A:N/A (2014). Human . PubMed: 25155877
- Takai S et al. Distinct effects of saracatinib on memory CD8+ T cell differentiation. J Immunol 188:4323-33 (2012). IP . PubMed: 22450814
- Randriamboavonjy V et al. AMPK {alpha}2 subunit is involved in platelet signaling, clot retraction, and thrombus stability. Blood 116:2134-40 (2010). WB ; Mouse . PubMed: 20558612
- Mitchell LA et al. Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa. Mol Hum Reprod 14:235-43 (2008). PubMed: 18245108
- Hauri-Hohl MM et al. Donor T-cell alloreactivity against host thymic epithelium limits T-cell development after bone marrow transplantation. Blood 109:4080-8 (2007). PubMed: 17213290