製品の概要

  • 製品名

    Anti-Fyn antibody [FYN-01]
    Fyn 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [FYN-01] to Fyn
  • 由来種

    Mouse
  • 特異性

    The antibody reacts with p59fyn (Fyn), a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases.
  • アプリケーション

    適用あり: IP, Flow Cyt, ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 7-176 of Human Fyn.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 59 kDa.

ターゲット情報

  • 機能

    Tyrosine-protein kinase implicated in the control of cell growth. Plays a role in the regulation of intracellular calcium levels, with isoform 2 showing the greater ability to mobilize cytoplasmic calcium in comparison to isoform 1. Required in brain development and mature brain function with important roles in the regulation of axon growth, axon guidance, and neurite extension. Blocks axon outgrowth and attraction induced by NTN1 by phosphorylating its receptor DDC. Phosphorylates RUNX3.
  • 組織特異性

    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T lymphocytes.
  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 細胞内局在

    Cell membrane. Present and active in lipid rafts. Present in cell body and along the process of mature and developing oligodendroyctes.
  • Information by UniProt
  • 参照データベース

  • 製品の状態

    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), YES1 (P07947), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • 別名

    • C syn protooncogene antibody
    • Fyn antibody
    • FYN oncogene related to SRC FGR YES antibody
    • FYN_HUMAN antibody
    • OKT3 induced calcium influx regulator antibody
    • P59 FYN antibody
    • p59-Fyn antibody
    • Protein tyrosine kinase fyn antibody
    • Proto oncogene tyrosine protein kinase fyn antibody
    • Proto-oncogene c-Fyn antibody
    • Proto-oncogene Syn antibody
    • Protooncogene Syn antibody
    • SLK antibody
    • Src like kinase antibody
    • Src yes related novel gene antibody
    • Src-like kinase antibody
    • Src/yes related novel antibody
    • SYN antibody
    • Tyrosine kinase p59fyn T antibody
    • Tyrosine kinase p59fyn(T) antibody
    • Tyrosine-protein kinase Fyn antibody
    see all

画像

  • Anti-Fyn antibody [FYN-01] (ab1881) at 1 µg/ml + Recombinant human Fyn protein (ab84696) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 59 kDa


    Exposure time: 10 seconds
  • Immunocytochemistry/ Immunofluorescence analysis of human primary fibroblasts labelling Fyn with ab1881 at 5 µL. Actin cytoskeleton was stained with phalloidin (red) and cell nuclei were stained with DAPI (blue). 
  • Overlay histogram showing Ramos cells stained with ab1881 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献

This product has been referenced in:

  • Yu L  et al. c-Maf inducing protein inhibits cofilin-1 activity and alters podocyte cytoskeleton organization. Mol Med Rep 16:4955-4963 (2017). Read more (PubMed: 28791377) »
  • Suo M  et al. Role of Fyn-mediated NMDA receptor function in prediabetic neuropathy in mice. J Neurophysiol 116:448-55 (2016). Read more (PubMed: 27146985) »
See all 9 Publications for this product

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your inquiry.
The image on the datasheet of ab1881 shows a WB with the positive control ab84696.
ab84696 is a Active human Fyn full length protein that has a tag. The tag has a size of ca. 26kDa.
Therefore the band at ca 85 kDa is what we would expect.
If you are using a sample with endogenous Fyn the expected size is ca 59 kDa.
I hope this information is helpful and wish you good luck with your research.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Huh7 (liver cell9)
Gel Running Conditions
Reduced Denaturing
Loading amount
200000 cells
Specification
Huh7 (liver cell9
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Mar 25 2019

Question
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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