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RabMAb

Anti-FYB 抗体 [EPR2547Y] (ab76006)

製品の概要

  • 製品名
    Anti-FYB antibody [EPR2547Y]
    FYB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2547Y] to FYB
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide within Human FYB aa 700-800 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • TF-1 lysate. Human tonsil tissue.
  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR2547Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC
  • 追加情報
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/500. Predicted molecular weight: 85 kDa.

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Acts as an adapter protein of the FYN and LCP2 signaling cascades in T cells. Modulates the expression of interleukin-2 (IL-2). Involved in platelet activation. Prevents the degradation of SKAP1 and SKAP2.
    • 組織特異性
      Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.
    • 配列類似性
      Contains 1 SH3 domain.
    • 翻訳後修飾
      T-cell receptor ligation leads to increased tyrosine phosphorylation.
    • 細胞内局在
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADAP antibody
      • Adhesion and degranulation promoting adaptor protein antibody
      • FYB 120/130 antibody
      • Fyb antibody
      • FYB-120/130 antibody
      • FYB_HUMAN antibody
      • FYN binding protein antibody
      • FYN T binding protein antibody
      • FYN-binding protein antibody
      • FYN-T-binding protein antibody
      • p120/p130 antibody
      • PRO0823 antibody
      • SLAP 130 antibody
      • SLAP-130 antibody
      • SLAP130 antibody
      • SLP 76 associated phosphoprotein antibody
      • SLP-76-associated phosphoprotein antibody
      • SLP76 associated phosphoprotein antibody
      see all

    画像

    • Anti-FYB antibody [EPR2547Y] (ab76006) at 1/500 dilution + TF-1 lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 85 kDa
      Observed band size: 120 kDa (why is the actual band size different from the predicted?)

    • ab76006, at 1/100 dilution, staining FYB in human tonsil by immunohistochemistry using paraffin-embedded tissue.

    参考文献

    This product has been referenced in:

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (CHO cell transfected)
    Loading amount
    20 µg
    Specification
    CHO cell transfected
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

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    Verified customer

    投稿 Jun 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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