Furin peptide (ab4989)
Key features and details
- Suitable for: Blocking
製品の詳細
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製品名
Furin peptide
Furin タンパク質・ペプチド 製品一覧 -
Animal free
No -
由来
Synthetic
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab4989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Blocking
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製品の状態
Lyophilized -
備考
This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and furin convertase, catalog ab3467. Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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再構成>95% pure, lyophilized synthetic peptide. Reconstitute with 0.1 ml of distilled water.
関連情報
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別名
- Dibasic processing enzyme
- Dibasic-processing enzyme
- FES upstream region
see all -
機能
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
組織特異性
Seems to be expressed ubiquitously. -
配列類似性
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
ドメイン
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
翻訳後修飾
The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab4989 は論文での使用が確認できていません。
