Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (3T3 fibroblasts)
Specification
3T3 fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TX-100
Blocking step
1% BSA/ 5%FBS/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 0.5% BSA/2.5% FBS in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probes
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/500
Additional data
Additional Notes
Cells were counterstained with DAPI (436nM, 5 mins) and Phalloidin-633 (1unit/coverslip) and mounted with prolong gold. No staining seen in secondary control and furin staining was punctate and throughout the cytoplasm.
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投稿 May 12 2012