Anti-Furin 抗体 (ab3467)
Key features and details
- Rabbit polyclonal to Furin
- Suitable for: WB
- Reacts with: Mouse, Sheep, Hamster, Dog, Human, Pig, Non human primates
- Isotype: IgG
製品の概要
-
製品名
Anti-Furin antibody
Furin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Furin -
由来種
Rabbit -
特異性
Detects Furin from canine and mouse cells as well as transfected human Furin. This antibody does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Sheep, Hamster, Dog, Human, Pig, Non human primates -
免疫原
Synthetic peptide corresponding to Human Furin aa 780-793.
Sequence:RGERTAFIKDQSAL
(Peptide available asab4989)
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).Can be blocked with Furin peptide (ab4989). Representing Furin in BSC-40 cells transfected with the human Furin gene. |
ターゲット情報
-
機能
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. -
組織特異性
Seems to be expressed ubiquitously. -
配列類似性
Belongs to the peptidase S8 family. Furin subfamily.
Contains 1 homo B/P domain. -
ドメイン
Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface. -
翻訳後修飾
The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin. - Information by UniProt
-
参照データベース
- Entrez Gene: 5045 Human
- Entrez Gene: 18550 Mouse
- Omim: 136950 Human
- SwissProt: P09958 Human
- SwissProt: P23188 Mouse
- Unigene: 513153 Human
- Unigene: 5241 Mouse
-
別名
- Dibasic processing enzyme antibody
- Dibasic-processing enzyme antibody
- FES upstream region antibody
see all
画像
-
Western blot - Anti-Furin antibody (ab3467)All lanes : Anti-Furin antibody (ab3467) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 90 seconds
The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide.
プロトコール
データシートおよび資料
参考文献 (14)
ab3467 は 14 報の論文で使用されています。
- Chang JC et al. SOST/Sclerostin Improves Posttraumatic Osteoarthritis and Inhibits MMP2/3 Expression After Injury. J Bone Miner Res 33:1105-1113 (2018). PubMed: 29377313
- Zhao G et al. Influence of a Coronary Artery Disease-Associated Genetic Variant on FURIN Expression and Effect of Furin on Macrophage Behavior. Arterioscler Thromb Vasc Biol 38:1837-1844 (2018). PubMed: 29976768
- Knab VM et al. Acute Parathyroid Hormone Injection Increases C-Terminal but Not Intact Fibroblast Growth Factor 23 Levels. Endocrinology 158:1130-1139 (2017). PubMed: 28324013
- Delestre-Delacour C et al. Myosin 1b and F-actin are involved in the control of secretory granule biogenesis. Sci Rep 7:5172 (2017). PubMed: 28701771
- Zhou Z et al. The cAMP-responsive element binding protein (CREB) transcription factor regulates furin expression during human trophoblast syncytialization. Placenta 35:907-18 (2014). PubMed: 25175744
