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  1. Link

    furin-antibody-ab3467.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-Furin 抗体 (ab3467)

  • Datasheet
Reviews (7)Q&A (2)References (14)

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Abpromise

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Immunocytochemistry - Anti-Furin antibody (ab3467)
  • Western blot - Anti-Furin antibody (ab3467)
  • Immunocytochemistry - Anti-Furin antibody (ab3467)

Key features and details

  • Rabbit polyclonal to Furin
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Sheep, Hamster, Dog, Human, Pig, Non human primates
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Recombinant Furin protein (ab6032)

関連製品

製品の概要

  • 製品名

    Anti-Furin antibody
    Furin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Furin
  • 由来種

    Rabbit
  • 特異性

    Detects Furin from canine and mouse cells as well as transfected human Furin. This antibody does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue.
  • アプリケーション

    適用あり: ICC, WBmore details
  • 種交差性

    交差種: Mouse, Sheep, Hamster, Dog, Human, Pig, Non human primates
  • 免疫原

    Synthetic peptide corresponding to Human Furin aa 780-793.
    Sequence:

    RGERTAFIKDQSAL


    (Peptide available as ab4989)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of Furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • Proprotein Convertases

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Furin peptide (ab4989)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Furin protein (ab6032)
  • Recombinant Protein

    • Recombinant Furin protein (ab6032)

アプリケーション

Our Abpromise guarantee covers the use of ab3467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).Can be blocked with Furin peptide (ab4989).

Representing Furin in BSC-40 cells transfected with the human Furin gene.

ターゲット情報

  • 機能

    Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.
  • 組織特異性

    Seems to be expressed ubiquitously.
  • 配列類似性

    Belongs to the peptidase S8 family. Furin subfamily.
    Contains 1 homo B/P domain.
  • ドメイン

    Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.
  • 翻訳後修飾

    The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.
    Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.
  • 細胞内局在

    Golgi apparatus > trans-Golgi network membrane. Cell membrane. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.
  • Target information above from: UniProt accession P09958 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5045 Human
    • Entrez Gene: 18550 Mouse
    • Omim: 136950 Human
    • SwissProt: P09958 Human
    • SwissProt: P23188 Mouse
    • Unigene: 513153 Human
    • Unigene: 5241 Mouse
    • 別名

      • Dibasic processing enzyme antibody
      • Dibasic-processing enzyme antibody
      • FES upstream region antibody
      • FUR antibody
      • FURIN antibody
      • Furin membrane associated receptor protein antibody
      • FURIN_HUMAN antibody
      • PACE antibody
      • Paired basic amino acid residue cleaving enzyme antibody
      • Paired basic amino acid residue-cleaving enzyme antibody
      • PCSK3 antibody
      • Proprotein convertase subtilisin/kexin type 3 antibody
      • SPC1 antibody
      see all

    画像

    • Immunocytochemistry - Anti-Furin antibody (ab3467)
      Immunocytochemistry - Anti-Furin antibody (ab3467)

      Immunolocalization of endogenous furin in mouse 3T3 cells with ab3467.

    • Western blot - Anti-Furin antibody (ab3467)
      Western blot - Anti-Furin antibody (ab3467)
      All lanes : Anti-Furin antibody (ab3467) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 87 kDa
      Observed band size: 63 kDa
      why is the actual band size different from the predicted?


      Exposure time: 90 seconds


      The band observed at 63 kDa could potentially be a cleaved form of Furin due to the presence of a 24 amino acid signal peptide and a 83 amino acid propeptide.
    • Immunocytochemistry - Anti-Furin antibody (ab3467)
      Immunocytochemistry - Anti-Furin antibody (ab3467)Image courtesy of an anonymous Abreview.
      ab3467 staining Furin in human A549 cells by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed with paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% BSA/ 5%FBS/PBS for 1 hour at 25°C and then incubated with ab3467 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

      Cells were counterstained with DAPI (436nM, 5 mins) and Phalloidin-633 (1unit/coverslip) and mounted with prolong gold. No staining seen in secondary control and furin staining was punctate and throughout the cytoplasm.

      See Abreview

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (14)

    ab3467 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3467 は 14 報の論文で使用されています。

    • Chang JC  et al. SOST/Sclerostin Improves Posttraumatic Osteoarthritis and Inhibits MMP2/3 Expression After Injury. J Bone Miner Res 33:1105-1113 (2018). PubMed: 29377313
    • Zhao G  et al. Influence of a Coronary Artery Disease-Associated Genetic Variant on FURIN Expression and Effect of Furin on Macrophage Behavior. Arterioscler Thromb Vasc Biol 38:1837-1844 (2018). PubMed: 29976768
    • Knab VM  et al. Acute Parathyroid Hormone Injection Increases C-Terminal but Not Intact Fibroblast Growth Factor 23 Levels. Endocrinology 158:1130-1139 (2017). PubMed: 28324013
    • Delestre-Delacour C  et al. Myosin 1b and F-actin are involved in the control of secretory granule biogenesis. Sci Rep 7:5172 (2017). PubMed: 28701771
    • Zhou Z  et al. The cAMP-responsive element binding protein (CREB) transcription factor regulates furin expression during human trophoblast syncytialization. Placenta 35:907-18 (2014). PubMed: 25175744
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-9 of 9 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Furin antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X100
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 02 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Furin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (INS1 cells)
    Permeabilization
    Yes - Triton X100 0.5%
    Specification
    INS1 cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20µg/mL · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 29 2020

    Western blot abreview for Anti-Furin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hek293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Hek293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 09 2020

    Western blot abreview for Anti-Furin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African green monkey Cell lysate - other (Vero)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Vero
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 08 2020

    Western blot abreview for Anti-Furin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HAP1)
    Gel Running Conditions
    Non-reduced Denaturing (4-20% tris-glycine)
    Loading amount
    250000 cells
    Specification
    HAP1
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Mr. Lawrence Welch

    Verified customer

    投稿 May 07 2020

    Question

    So, you are saying, the 60kD band is the Signal Sequence (˜24AA)+ Propeptide (˜130AA) + catalytic domain (˜280)+ partial RGD domain.



    Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2012

    Answer

    Sorry for this misunderstanding.

    We believe that the antibody recognizes the mature form of the protein. The pro-peptide is expected at 87kDa and once the signal and pro-peptide are cleaved off the protein, it migrates at 63 kDa.

    I hope this is helpful and wish you a good weekend.

    Read More

    Abcam Scientific Support

    Answered on May 18 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Furin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (3T3 fibroblasts)
    Specification
    3T3 fibroblasts
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TX-100
    Blocking step
    1% BSA/ 5%FBS/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 12 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Furin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549 lung epithelial cell line)
    Specification
    A549 lung epithelial cell line
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TX-100
    Blocking step
    1% BSA/ 5%FBS/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 12 2012

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 111548 DESCRIPTION OF THE PROBLEM Antibody recognises multiple bands - major ones at 250, 100, 75 (triplet) and also picks up 37 kDa MW marker! Peptide (ab4989) takes out 250, upper band of 75 kDa triplet and MW marker (!). SAMPLE Human cultured cardiac fibroblasts - whole cell homogenate PRIMARY ANTIBODY Abcam furin convertase (ab3467) 1:500 - 1:1000 DETECTION METHOD Pierce ECL POSITIVE AND NEGATIVE CONTROLS USED HT-1080 cell homogenate ANTIBODY STORAGE CONDITIONS -20C aliquoted SAMPLE PREPARATION Laemmli sample buffer + PMSF + Na vanadate. Boiled + 2-mercaptoethanol for 5 min. AMOUNT OF PROTEIN LOADED 50-100 ug per lane ELECTROPHORESIS/GEL CONDITIONS 10% gel. reducing. TRANSFER AND BLOCKING CONDITIONS Blocked in 5% Milk in TBS Tween SECONDARY ANTIBODY [a competitor] rabbit-HRP 1:2000 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Antibody dilution, addition of neutralising peptide ADDITIONAL NOTES Attached is a figure of blot with and without peptide.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 31 2006

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We have received a favourable review of our Furin Convertase antibody (ab3467) albeit with acknowledgement of the detection of some non-specific bands. We often receive complaints relating to aberrant bands that can often be resolved with the use of 5% BSA as a blocking agent. We find it gives a cleaner more specific blot. I would therefore like to recommend its use on this occasion. I would also recommend performing an overnight incubation of the antibody, at a more dilute concentration e.g. 1:1500 at 4oC as again we often find that this increases the specificity of the blot. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 01 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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