Anti-FUNDC1 抗体 (ab272627)
Key features and details
- Rabbit polyclonal to FUNDC1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FUNDC1 antibody
FUNDC1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FUNDC1 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
免疫原
Recombinant fragment corresponding to Human FUNDC1 aa 1-100.
Database link: Q8IVP5 -
ポジティブ･コントロール
- WB: HEK-293T cell lysate. IHC-P: Human prostate tissue, cerebral tissue, stomach tissue, liver tissue.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Cow
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the FUN14 family. -
ドメイン
The YXXL motif mediates the interaction with MAP1 LC3 family proteins MAP1LC3A, MAP1LC3B and GABARAP (PubMed:22267086). -
翻訳後修飾
Phosphorylation at Tyr-18 by SRC inhibits activation of mitophagy. Following hypoxia, dephosphorylated at Tyr-18, leading to interaction with MAP1 LC3 family proteins and triggering mitophagy. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 518258 Cow
- Entrez Gene: 139341 Human
- Entrez Gene: Mouse
- Entrez Gene: 72018 Mouse
- Entrez Gene: 363442 Rat
- Omim: 300871 Human
- SwissProt: F1N5S9 Cow
- SwissProt: Q8IVP5 Human
see all -
別名
- FUN14 domain containing 1 antibody
- FUN14 domain containing protein 1 antibody
- FUN14 domain-containing protein 1 antibody
see all
画像
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Western blot - Anti-FUNDC1 antibody (ab272627)All lanes : Anti-FUNDC1 antibody (ab272627) at 0.4 µg/ml
Lane 1 : Control (vector only transfected HEK-293T lysate)
Lane 2 : UNDC1 over-expression lysate (mammalian HEK-293T cells) -
Western blot - Anti-FUNDC1 antibody (ab272627)Anti-FUNDC1 antibody (ab272627) at 0.4 µg/ml + HEK-293 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (ab272627)Paraffin-embedded formalin-fixed Human prostate tissue stained for FUNDC1 using ab272627 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (ab272627)Paraffin-embedded formalin-fixed Human cerebral tissue stained for FUNDC1 using ab272627 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (ab272627)Paraffin-embedded formalin-fixed Human stomach tissue stained for FUNDC1 using ab272627 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUNDC1 antibody (ab272627)Paraffin-embedded formalin-fixed Human liver tissue stained for FUNDC1 using ab272627 at 1/500 in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272627 は論文での使用が確認できていません。
