Anti-FUBP1/FBP 抗体 [EPR12327] (ab181111)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12327] to FUBP1/FBP
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FUBP1/FBP antibody [EPR12327]
FUBP1/FBP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12327] to FUBP1/FBP -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Jurkat, HeLa, Raji and HepG2 whole cell lysate (ab7900), rat brain, mouse brain and mouse spleen tissue lysates. IHC-P: Human breast carcinoma and pancreas tissues. ICC/IF: HeLa and HepG2 cells. Flow Cyt (intra): Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12327 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- Mouse spleen tissue lysate - total protein (ab29293)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse spleen tissue lysate - total protein (14 days) (ab7274)
- Jurkat whole cell lysate (ab7899)
- Mouse small intestine tissue lysate - total protein (ab7939)
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181111の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/60 - 1/90.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa).
For unpurified use at 1/1000 - 1/20000. |
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/250 - 1/500.
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特記事項 |
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Flow Cyt (Intra)
1/60 - 1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/2000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa). For unpurified use at 1/1000 - 1/20000. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
ターゲット情報
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機能
Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. -
配列類似性
Contains 4 KH domains. -
翻訳後修飾
Ubiquitinated. This targets the protein for proteasome-mediated degradation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8880 Human
- Entrez Gene: 51886 Mouse
- Entrez Gene: 654496 Rat
- Omim: 603444 Human
- SwissProt: Q96AE4 Human
- SwissProt: Q91WJ8 Mouse
- SwissProt: Q32PX7 Rat
- Unigene: 567380 Human
see all -
別名
- DNA helicase V antibody
- far upstream element (FUSE) binding protein 1 antibody
- Far upstream element (FUSE) binding protein 4 antibody
see all
画像
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All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/2000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/20000 dilution (purified)
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FUBP1/FBP with purified ab181111 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FUBP1/FBP with purified ab181111 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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Intracellular Flow Cytometry analysis of Jurkat cells labelling FUBP1 / FBP with purified ab181111 at 1/60 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/5000 dilution (unpurified) + Jurkat cell lysate at 20 µg
Secondary
Goat anti-rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted? -
All lanes : Anti-FUBP1/FBP antibody [EPR12327] (ab181111) at 1/20000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/500 followed by prediluted HRP Polymer for rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/500 followed by prediluted HRP Polymer for rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling FUBP1/FBP with unpurified ab181111 at 1/500 dilution (red) followed by Alexa Fluor® 555-conjugated goat anti rabbit IgG secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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Intracellular flow cytometric analysis of2% paraformaldehyde-fixed Jurkat cells labeling FUBP1/FBP with unpurified ab181111 at a dilution of 1/90 (pink), compared to a rabbit monoclonal IgG isotype control (green), followed by goat anti rabbit IgG (FITC) secondary antibody at a dilution of 1/150.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (12)
ab181111 は 12 報の論文で使用されています。
- Olguin SL et al. KHSRP loss increases neuronal growth and synaptic transmission and alters memory consolidation through RNA stabilization. Commun Biol 5:672 (2022). PubMed: 35798971
- Yang B et al. TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical Cancer. J Immunol Res 2021:9994004 (2021). PubMed: 33987449
- Wang H et al. Protein and Signaling Pathway Responses to rhIL-6 Intervention Before Lobaplatin Treatment in Osteosarcoma Cells. Front Oncol 11:602712 (2021). PubMed: 33791202
- Wen H et al. Long non-coding RNA KRT8P41/miR-193a-3p/FUBP1 axis modulates the proliferation and invasion of chordoma cells. J Bone Oncol 31:100392 (2021). PubMed: 34712553
- Zhang XX et al. Long non-coding RNA small nucleolar RNA host gene 6 aggravates pancreatic cancer through upregulation of far upstream element binding protein 1 by sponging microRNA-26a-5p. Chin Med J (Engl) 133:1211-1220 (2020). PubMed: 32433053