Fructose Assay Kit (ab83380)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture extracts, Tissue Extracts
製品の概要
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製品名
Fructose Assay Kit
Fructose キット 製品一覧 -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Quantitative -
全工程の試験時間
1h 00m -
製品の概要
In Abcam's Fructose Assay Kit, free fructose is enzymatically converted to ß-glucose, which is then specifically converted to a product that reacts with OxiRed Probe to generate color (λ=570nm) and fluorescence (Ex/Em=535/587nm). The kit provides a rapid, simple, sensitive, and reliable method suitable for high throughput assayof D-fructose. Do not use this kit with urine, serum, or plasma samples.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K619 Fructose Colorimetric/Fluorometric Assay Kit. K619-100 is the same size as the 100 test size of ab83380.
Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Development Enzyme Mix II 1 vial Fructose Assay Buffer 1 x 25ml Fructose Converter Mix 1 vial Fructose Standard 1 x 100µl OxiRed Probe 1 x 200µl -
研究分野
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関連性
Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose. -
別名
- Beta Levulose
- D arabino Hexulose
- Fruit sugar
see all
画像
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Fructose levels banana, determined using the colorimetric (C) and the fluorometric (F) method (presented per mg of protein) (duplicates; +/- SD).
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Fructose levels in mouse liver and banana, determined using the colorimetric method and presented per mg of protein (duplicates; +/- SD).
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Titration of the fructose (background subtracted, duplicates; +/- SD).
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Fluorometric Fructose Standard Curve performed following the ab83380 kit protocol.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab83380 は 10 報の論文で使用されています。
- Huang X et al. IL-6/STAT3 Axis Activates Glut5 to Regulate Fructose Metabolism and Tumorigenesis. Int J Biol Sci 18:3668-3675 (2022). PubMed: 35813468
- Sakiyama H et al. ChREBP deficiency prevents high sucrose diet-induced obesity through reducing sucrase expression. J Clin Biochem Nutr 71:221-228 (2022). PubMed: 36447485
- Gungor A et al. The Relationship between Daily Fructose Consumption and Oxidized Low-Density Lipoprotein and Low-Density Lipoprotein Particle Size in Children with Obesity. Pediatr Gastroenterol Hepatol Nutr 24:483-491 (2021). PubMed: 34557400
- Maslova O et al. Production of various organic acids from different renewable sources by immobilized cells in the regimes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SFF). Bioresour Technol 272:1-9 (2019). PubMed: 30292911
- Aw WC et al. Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness. PLoS Genet 14:e1007735 (2018). PubMed: 30399141
- Gonzalez-Vicente A et al. FRUCTOSE REABSORPTION BY RAT PROXIMAL TUBULES: ROLE OF SODIUM-LINKED COTRANSPORTERS AND THE EFFECT OF DIETARY FRUCTOSE. Am J Physiol Renal Physiol N/A:N/A (2018). PubMed: 30565998
- Vaughan OR et al. A physiological increase in maternal cortisol alters uteroplacental metabolism in the pregnant ewe. J Physiol 594:6407-6418 (2016). PubMed: 27292274
- Romero Mdel M et al. Glycerol Production from Glucose and Fructose by 3T3-L1 Cells: A Mechanism of Adipocyte Defense from Excess Substrate. PLoS One 10:e0139502 (2015). Functional Studies . PubMed: 26426115
- Cheng CY et al. Borate-fructose complex: A novel mediator for laccase and its new function for fructose determination. FEBS Lett 589:3107-12 (2015). PubMed: 26335748
- Wilkens CA & Gerdtzen ZP Comparative metabolic analysis of CHO cell clones obtained through cell engineering, for IgG productivity, growth and cell longevity. PLoS One 10:e0119053 (2015). PubMed: 25768021