Fructose-6-Phosphate Assay Kit (Fluorometric) (ab204720)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 1 µM
製品の概要
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製品名
Fructose-6-Phosphate Assay Kit (Fluorometric) -
検出方法
Fluorescent -
サンプルの種類
Cell Lysate, Tissue Lysate -
検出感度
1 µM -
製品の概要
Fructose-6-Phosphate Assay Kit (ab204720) is a fluorescence based simple, highly sensitive and rapid means of quantifying fructose-6-phosphate (F6P) in a variety of samples. In the assay, F6P is converted to glucose-6-phosphate (G6P) which is subsequently oxidized with the generation of a fluorescent product. This assay kit can detect F6P in the range of 0.01 to 0.5 nmoles with detection sensitivity ∼ 1 µM of F6P.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K689 PicoProbe™ Fructose-6-Phosphate Fluorometric Assay Kit. K689-100 is the same size as the 100 test size of ab204720.
Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer V 1 x 25ml Converter Enzyme X 1 vial Developer IX 1 vial Development Enzyme Mix IX 1 vial F6P Standard 1 vial PicoProbe I 1 x 400µl
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab204720 は 8 報の論文で使用されています。
- Wang W & Wang B KDM3A-mediated SP1 activates PFKFB4 transcription to promote aerobic glycolysis in osteosarcoma and augment tumor development. BMC Cancer 22:562 (2022). PubMed: 35590288
- Yoo HJ et al. MsrB1-regulated GAPDH oxidation plays programmatic roles in shaping metabolic and inflammatory signatures during macrophage activation. Cell Rep 41:111598 (2022). PubMed: 36351405
- Huangyang P et al. Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis. Cell Metab 31:174-188.e7 (2020). PubMed: 31761563
- Liao K et al. A Feedback Circuitry between Polycomb Signaling and Fructose-1, 6-Bisphosphatase Enables Hepatic and Renal Tumorigenesis. Cancer Res 80:675-688 (2020). PubMed: 31948940
- Zhao P et al. Suppressed "Warburg Effect" in Nasopharyngeal Carcinoma Via the Inhibition of Pyruvate Kinase Type M2-Mediated Energy Generation Pathway. Technol Cancer Res Treat 19:1533033820945804 (2020). PubMed: 32815467
- Chen Y & Zhu W Knockdown of the Sonic Hedgehog (SHH) Gene Inhibits Proliferation of Hep3B and SMMC-7721 Hepatocellular Carcinoma Cells via the PI3K/Akt/PCK1 Signaling Pathway. Med Sci Monit 25:6023-6033 (2019). PubMed: 31406102
- Tang Y et al. Overexpression of PCK1 Gene Antagonizes Hepatocellular Carcinoma Through the Activation of Gluconeogenesis and Suppression of Glycolysis Pathways. Cell Physiol Biochem 47:344-355 (2018). PubMed: 29768256
- Geng H et al. Interplay between hypoxia and androgen controls a metabolic switch conferring resistance to androgen/AR-targeted therapy. Nat Commun 9:4972 (2018). PubMed: 30478344