Anti-FREM1 抗体 (ab105360)
Key features and details
- Rabbit polyclonal to FREM1
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FREM1 antibody
FREM1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FREM1 -
由来種
Rabbit -
特異性
ab105360 is predicted to not crossreact with other FRAS members. Multiple isoforms are known to exist. -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Human -
免疫原
A 15 amino acid peptide near the C terminus of Human FREM1 (NP_659403).
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ポジティブ･コントロール
- K562 cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105360の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml.
Predciated Band: 244 for isoform 1 , for isoform 2 (TILRR), 80 kDa |
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| ICC |
Use a concentration of 20 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Predciated Band: 244 for isoform 1 , for isoform 2 (TILRR), 80 kDa |
|
ICC
Use a concentration of 20 µg/ml. |
ターゲット情報
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機能
Extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. -
関連疾患
Defects in FREM1 are the cause of bifid nose with or without anorectal and renal anomalies (BNAR) [MIM:608980]. A bifid nose is a rare congenital deformity due to failure of the paired nasal processes to fuse to a single midline organ during early gestation. BNAR is an autosomal recessive disorder and patients usually present a bifid nose associated with renal and anorectal malformations. -
配列類似性
Belongs to the FRAS1 family.
Contains 1 C-type lectin domain.
Contains 1 Calx-beta domain.
Contains 12 CSPG (NG2) repeats. -
ドメイン
The Calx-beta domain binds calcium with high affinity and undergo a major conformational shift upon binding. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. Localizes at the basement membrane zone of embryonic epidermis and hair follicles. - Information by UniProt
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参照データベース
- Entrez Gene: 158326 Human
- Omim: 608944 Human
- SwissProt: Q5H8C1 Human
- Unigene: 50850 Human
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別名
- BC037594 antibody
- BNAR antibody
- C9orf143 antibody
see all
画像
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Immunocytochemistry - Anti-FREM1 antibody (ab105360)
Immunofluorescence of FREM1 in K562 cells with ab105360 at 20 ug/mL.
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Western blot - Anti-FREM1 antibody (ab105360)Lane 1 : Anti-FREM1 antibody (ab105360) at 1 µg/ml
Lane 2 : Anti-FREM1 antibody (ab105360) at 0.5 µg/ml
All lanes : K562 cell lysate
Lysates/proteins at 15 µg per lane.Predicted band size: 244 for isoform 1 , for isoform 2 (TILRR), 80 kDa
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Immunocytochemistry - Anti-FREM1 antibody (ab105360)Immunocytochemistry of FREM1 in K562 cells with ab105360 at 20 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab105360 は論文での使用が確認できていません。
