製品の概要

  • 製品名
    Free Glycerol Assay Kit II (Colorimetric)
    Glycerol キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
  • アッセイタイプ
    Quantitative
  • 検出感度
    < 20 µM
  • 検出範囲
    2 nmol/well - 10 nmol/well
  • 全工程の試験時間
    1h 30m
  • 種交差性
    交差種: Mammals, Other species
  • 製品の概要

    Abcam’s Free Glycerol Assay kit II (ab155899) is suitable for measuring free glycerol levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, Glycerol in the presence of Glycerol Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. Free Glycerol Assay Kit II is simple, rapid and high-throughput adaptable. This assay kit can detect less than 20 μM of free Glycerol in various biological samples.


    Detection method: colorimetric – based on enzyme activity

  • 特記事項

    Glycerol is a central component of most lipids. It acts as a backbone for triglycerides and phospholipids, which play an important role in metabolism and cell membrane structure. Due to its low toxicity, glycerol is widely used in the pharmaceutical, food and cosmetic industries.

  • 試験プラットフォーム
    Microplate reader

製品の特性

画像

  • Glycerol Standard Curve [a]. Measurement of free glycerol in bovine liver (50 µg), Jurkat cells (40 µg), HeLa cells (30 µg), fish egg (58 µg) & human serum (2 µl) [b]. Assays were performed following kit protocol.

プロトコール

参考文献

ab155899 has not yet been referenced specifically in any publications.

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