Free Glycerol Assay Kit (ab65337)
製品の概要
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製品名
Free Glycerol Assay Kit
Glycerol キット 製品一覧 -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Quantitative -
検出感度
> 1 µM -
検出範囲
1 µM - 10000 µM -
全工程の試験時間
0h 40m -
製品の概要
Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.
In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol.
Glycerol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 100 tests Glycerol Assay Buffer WM 1 x 25ml Glycerol Enzyme Mix (lyophilized) Green 1 vial Glycerol Probe (in DMSO, anhydrous) Red 1 x 200µl Glycerol Standard (100 mM) Yellow 1 x 200µl -
研究分野
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関連性
Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development. -
別名
- Glycerin
- Glycerine
関連製品
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Related Products
画像
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-8 fold and measured fluorometrically.
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Free Glycerol measured in biological fluids showing quantity (nmol) per mL of tested sample. Samples were diluted 2-8 fold and measured colorimetrically.
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a. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).
プロトコール
参考文献
This product has been referenced in:
- Mukherjee S et al. Derivation and characterization of a UCP1 reporter human ES cell line. Stem Cell Res 30:12-21 (2018). Read more (PubMed: 29777802) »
- Seki T et al. Ablation of endothelial VEGFR1 improves metabolic dysfunction by inducing adipose tissue browning. J Exp Med 215:611-626 (2018). Read more (PubMed: 29305395) »