Anti-FRAT1 抗体 [EPR3900] (ab108405)
![Western blot - Anti-FRAT1 antibody [EPR3900] (ab108405)](https://www.abcam.co.jp/ps/products/108/ab108405/Images/ab108405-121097-anti-frat1-antibody-epr3900-western-blot.jpg "Western blot - Anti-FRAT1 antibody [EPR3900] (ab108405)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3900] to FRAT1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FRAT1 antibody [EPR3900]
FRAT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3900] to FRAT1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa and MCF7 cell lysates ICC/IF: HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3900 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108405の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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| ICC/IF |
1/250.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
|
ICC/IF
1/250. |
ターゲット情報
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関連性
The protein encoded by the FRAT1 gene belongs to the GSK-3-binding protein family. The protein inhibits GSK-3-mediated phosphorylation of beta-catenin and also positively regulates the Wnt signaling pathway. It may play a role in tumor progression and in lymphomagenesis. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 10023 Human
- Entrez Gene: 14296 Mouse
- Omim: 602503 Human
- SwissProt: Q92837 Human
- SwissProt: P70339 Mouse
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別名
- FRAT 1 antibody
- frequently rearranged in advanced T cell lymphomas antibody
- Frequently rearranged in advanced T-cell lymphomas antibody
see all
画像
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Western blot - Anti-FRAT1 antibody [EPR3900] (ab108405)All lanes : Anti-FRAT1 antibody [EPR3900] (ab108405) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-FRAT1 antibody [EPR3900] (ab108405)](https://www.abcam.co.jp/ps/products/108/ab108405/Images/ab108405-2-anti-frat1-antibody-epr3900-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FRAT1 antibody [EPR3900] (ab108405)Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling FRAT1 with purified ab108405 at 1/250 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Anti-FRAT1 antibody [EPR3900] (ab108405)](https://www.abcam.co.jp/ps/products/108/ab108405/Images/ab108405-3-benefits-of-recombinant-antibodies.png)
Anti-FRAT1 antibody [EPR3900] (ab108405)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab108405 は 7 報の論文で使用されています。
- Guo G et al. STAT1 is a modulator of the expression of frequently rearranged in advanced T-cell lymphomas 1 expression in U251 cells. Oncol Lett 20:248-256 (2020). PubMed: 32565951
- Li B et al. Network-Wide Screen Identifies Variation of Novel Precise On-Module Targets Using Conformational Modudaoism. CPT Pharmacometrics Syst Pharmacol 7:16-25 (2018). IP . PubMed: 28925077
- Werner TV et al. MiR-34a-3p alters proliferation and apoptosis of meningioma cells in vitro and is directly targeting SMAD4, FRAT1 and BCL2. Aging (Albany NY) 9:932-954 (2017). WB . PubMed: 28340489
- Zhu K et al. FRAT1 expression regulates proliferation in colon cancer cells. Oncol Lett 12:4761-4766 (2016). PubMed: 28101222
- Zhang W et al. Frequently rearranged in advanced T-cell lymphomas-1 demonstrates oncogenic properties in prostate cancer. Mol Med Rep 14:3551-8 (2016). WB . PubMed: 27599661
- Yu Q et al. Silencing of FRAT1 by siRNA inhibits the proliferation of SGC7901 human gastric adenocarcinoma cells. Biomed Rep 4:223-226 (2016). PubMed: 26893843
- Jin R et al. The metastasis suppressor NDRG1 modulates the phosphorylation and nuclear translocation of ß-catenin through mechanisms involving FRAT1 and PAK4. J Cell Sci 127:3116-30 (2014). Human . PubMed: 24829151
