Anti-FOXP4 抗体 [EPR22714-2] (ab242127)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22714-2] to FOXP4
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Fr, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FOXP4 antibody [EPR22714-2]
FOXP4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22714-2] to FOXP4 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Fr, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2, Caco-2, HeLa, Hepa1-6, 4T1 and PC-12 whole cell lysates; Human colon and tonsil tissue lysates; Rat spleen tissue lysate. IHC-P: Human tonsil tissue; Mouse spleen tissue; Rat spleen tissue. IHC-Fr: Mouse colon tissue. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22714-2 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab242127の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/50.
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WB |
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).
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IP |
1/30.
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IHC-Fr |
1/1000.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
|
ICC/IF |
1/500.
|
特記事項 |
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Flow Cyt (Intra)
1/50. |
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa). |
IP
1/30. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
1/1000. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ICC/IF
1/500. |
ターゲット情報
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機能
Transcriptional repressor that represses lung-specific expression. -
配列類似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
ドメイン
The leucine-zipper is required for dimerization and transcriptional repression. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 116113 Human
- Entrez Gene: 74123 Mouse
- Entrez Gene: 363185 Rat
- Omim: 608924 Human
- SwissProt: Q5W098 Human
- SwissProt: Q8IVH2 Human
- SwissProt: Q80V92 Mouse
- SwissProt: Q8CG10 Mouse
see all -
別名
- 1200010K03Rik antibody
- 2310007G05Rik antibody
- FKHLA antibody
see all
画像
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All lanes : Anti-FOXP4 antibody [EPR22714-2] (ab242127) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 2 : FOXP4 knock out HepG2 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The lysates were kindly provided by our collaborator Dr. Bin Zhao, Zhejiang University.
Lanes 1-2: Merged signal (red and green). Green - ab242127 observed at 80 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
Lanes 1-2: ab242127 Anti-FOXP4 antibody [EPR22714-2] was shown to react with FOXP4 in HepG2 cells in Western blot. Loss of signal was observed when FOXP4 knockout sample was used. Wild-type and FOXP4 knockout samples were subjected to SDS-PAGE. ab242127 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-FOXP4 antibody [EPR22714-2] (ab242127) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure time: Lanes 1-2: 59 seconds; Lanes 3-4: 3 minutes.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP4 with ab242127 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human tonsil. The section was incubated with ab242127 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument, Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labelling FOXP4 with ab242127 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HepG2 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling FOXP4 with ab242127 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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FOXP4 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab242127 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242127 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab242127 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242127 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
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All lanes : Anti-FOXP4 antibody [EPR22714-2] (ab242127) at 1/1000 dilution
Lane 1 : Human colon tissue lysate
Lane 2 : Human tonsil tissue lysate
Lane 3 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Lane 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Bands around 50 kDa and 37 kDa in lane 3 are caused by degradation.
Exposure time: 3 minutes
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling FOXP4 with ab242127 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse spleen. The section was incubated with ab242127 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling FOXP4 with ab242127 at 1/1000 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Nuclear staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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All lanes : Anti-FOXP4 antibody [EPR22714-2] (ab242127) at 1/1000 dilution
Lane 1 : 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure time: 3 minutes
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Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling FOXP4 with ab242127 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat spleen. The section was incubated with ab242127 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab242127 は 1 報の論文で使用されています。
- Shi J et al. FOXP4 promotes laryngeal squamous cell carcinoma progression through directly targeting LEF‑1. Mol Med Rep 24:N/A (2021). PubMed: 34590150