Name: Anti-FOXP3 antibody [SP97]
Brand: RabMAb
SKU: ab99963
Rating: 1 (2 reviews)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

Loading size & price…

Abpromise

保証された製品品質、優れたカスタマー・サポート。詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP97] to FOXP3
Suitable for: IHC-P
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品名

Anti-FOXP3 antibody [SP97]
FOXP3 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [SP97] to FOXP3
由来種

Rabbit
アプリケーション

適用あり: IHC-Pmore details
種交差性

交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- IHC-P: Human tonsil, pancreatic adenocarcinoma, stomach adenocarcinoma, lymph node, B cell lymphoma, colon, bladder, bladder transitional cell carcinoma, colon adenocarcinoma, thymus and cervical squamous cell carcinoma.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
バッファー

pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

SP97
アイソタイプ

IgG
研究分野

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human FOXP3 protein (His tag) (ab226445)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab99963の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P	(2)	1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Boil tissue section in 10mM Citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min.

特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Boil tissue section in 10mM Citrate, pH 6.0 for 10 min followed by cooling at RT for 20 min.

機能

Probable transcription factor. Plays a critical role in the control of immune response.
関連疾患

Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
配列類似性

Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
細胞内局在

Nucleus.
Target information above from: UniProt accession Q9BZS1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 506053 Cow
- Entrez Gene: 491876 Dog
- Entrez Gene: 50943 Human
- Entrez Gene: 20371 Mouse
- Entrez Gene: 444998 Pig
- Entrez Gene: 317382 Rat
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- SwissProt: Q99JB6 Mouse
- Unigene: 247700 Human
- Unigene: 182291 Mouse
see all
別名
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
- Forkhead box protein P3 antibody
- FOXP3 antibody
- FOXP3_HUMAN antibody
- FOXP3delta7 antibody
- Immune dysregulation polyendocrinopathy enteropathy X linked antibody
- Immunodeficiency polyendocrinopathy enteropathy X linked antibody
- IPEX antibody
- JM2 antibody
- MGC141961 antibody
- MGC141963 antibody
- OTTHUMP00000025832 antibody
- OTTHUMP00000025833 antibody
- OTTHUMP00000226737 antibody
- PIDX antibody
- Scurfin antibody
- XPID antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP3 with ab99963, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on a subset of T cells in the human tonsil. The section was incubated with ab229902 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Immunohistochemical analysis of Human Tonsil tissue labelling FOXP3 with ab99963.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human B cell lymphoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human colon tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human bladder tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human thymus tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (ab99963)

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for FOXP3 using ab99963 at 1/100 dilution in immunohistochemical analysis.
Anti-FOXP3 antibody [SP97] (ab99963)

Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab99963 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab99963 は 17 報の論文で使用されています。

Jia X et al. Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome. Oncol Lett 19:442-448 (2020). PubMed: 31897157
Karimi M et al. The relation between the ghrelin receptor and FOXP3 in bladder cancer. Biotech Histochem N/A:1-9 (2020). PubMed: 32744468
Kanno H et al. Differences in the immunosurveillance pattern associated with DNA mismatch repair status between right-sided and left-sided colorectal cancer. Cancer Sci 111:3032-3044 (2020). PubMed: 32449240
Qin S et al. CD4+CD25+Foxp3+ regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-?B pathway. J Int Med Res 48:300060520980940 (2020). PubMed: 33356705
Feng M et al. Pharmacological inhibition of ß-catenin/BCL9 interaction overcomes resistance to immune checkpoint blockades by modulating Treg cells. Sci Adv 5:eaau5240 (2019). PubMed: 31086813

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Pig Tissue sections (skin, subcutaneous fat, lymphocytes)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6

Permeabilization

Yes - H2O2

Specification

skin, subcutaneous fat, lymphocytes

Blocking step

ab64226 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C

Fixative

10 Neutral Buffer Formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 04 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Mouse Spleen)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Ventana CC1 Solution

Permeabilization

Specification

Mouse Spleen

Blocking step

(agent) for 12 minute(s) · Concentration: 10% · Temperature: 37°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Jenna Vandervolgen

Verified customer

投稿 Aug 13 2018

Question

What fixative was used to produce the IHC-P image of Human Tonsil Samples, stained with product Anti-FOXP3 antibody [SP97] (ab99963)?

Abcam community

Verified customer

Asked on Oct 04 2011

Answer

For IHC-P staining with product ab99963, Human Tonsil Samples were formalin fixed.

Our full IHC-P fixation protocol can be found here: IHC Fixation Protocol.

Abcam Scientific Support

Answered on Oct 04 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (17)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [SP97]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [SP97]