Anti-FOXP3 抗体 [mAbcam 450] (ab450)

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製品名

Anti-FOXP3 antibody [mAbcam 450]
FOXP3 一次抗体製品一覧
製品の詳細

Mouse monoclonal [mAbcam 450] to FOXP3
由来種

Mouse
特異性

Detects a single clean band on human FOXP3 transfected 293 cells. When used in immunocytochemistry on acetone fixed FOXP protein transfected COS cells the antibody only detects FOXP3 and not FOXP1, FOXP2 or FOXP4.
アプリケーション

適用あり: IHC-Fr, IP, ICC, IHC-P, ICC/IF, WBmore details
種交差性

交差種: Human
免疫原

Synthetic peptide corresponding to Human FOXP3 aa 400 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q9BZS1
(Peptide available as ab16809)
ポジティブ･コントロール
- IHC-P: FFPE Human tonsil tissue sections. IHC-Fr: Human tonsil tissue sections.
特記事項

This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
精製度

IgG fraction
ポリ/モノ

モノクローナル
クローン名

mAbcam 450
ミエローマ

Sp2/0-Ag14
アイソタイプ

IgG3
軽鎖の種類

kappa
研究分野

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Immunizing Peptide (Blocking)
- Human FOXP3 peptide (ab16809)

Our Abpromise guarantee covers the use of ab450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
IHC-Fr		Use a concentration of 5 µg/ml.
IP		Use at an assay dependent concentration. Used at 2 µg/ml on human FOXP3 transfected COS cells (see Abreview for further information).
ICC		Use at an assay dependent concentration.
IHC-P		1/50. This clone has been tested against FOXP3 transfectants of COS-1 cells fixed with acetone and also works against formalin-fixed paraffin-embedded FOXP3 transfectants heat retrieved with pH9 Tris-EDTA buffer. It did not show positive staining with cryostat or paraffin-embedded human tonsil.
ICC/IF		Use at an assay dependent concentration.
WB		Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human FOXP3 peptide (ab16809).

機能

Probable transcription factor. Plays a critical role in the control of immune response.
関連疾患

Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.
配列類似性

Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
細胞内局在

Nucleus.
Target information above from: UniProt accession Q9BZS1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
別名
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
- Forkhead box protein P3 antibody
- FOXP3 antibody
- FOXP3_HUMAN antibody
- FOXP3delta7 antibody
- Immune dysregulation polyendocrinopathy enteropathy X linked antibody
- Immunodeficiency polyendocrinopathy enteropathy X linked antibody
- IPEX antibody
- JM2 antibody
- MGC141961 antibody
- MGC141963 antibody
- OTTHUMP00000025832 antibody
- OTTHUMP00000025833 antibody
- OTTHUMP00000226737 antibody
- PIDX antibody
- Scurfin antibody
- XPID antibody
see all

Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)

IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BOND^TM system using the standard protocol F. The section was then incubated with ab450, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 450] (ab450)

IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab450, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450)This image is courtesy of Alison Banham and Bridget Fox, University of Oxford

Clone mAbcam 450 used in immunocytochemistry on transfected COS-1 cytospins fixed with acetone for 10 mins.
1: FOXP1 transfectant
2: FOXP2 transfectant
3: FOXP3 transfectant
4: FOXP4 transfectant

Staining can be seen on the FOXP3 transfectant, but no staining is seen with the other FOXP protein transfectants, confirming the specificity of this antibody in immunocytochemistry.

These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).
Immunocytochemistry - Anti-FOXP3 antibody [mAbcam 450] (ab450)This image is courtesy of Alison Banham and Bridget Fox, University of Oxford

Clone mAbcam 450 used in immunocytochemistry on paraffin embedded transfected COS-1 cytospins.
1: FOXP1 transfectant
2: FOXP2 transfectant
3: FOXP3 transfectant
4: FOXP4 transfectant

Strong staining can be seen on the FOXP3 transfectant, with very weak staining on the FOXP1 transfectant and no staining is seen with the other FOXP protein transfectants. This shows that the antibody is largely specific for FOXP3 when used with paraffin embedded cells.

These images were produced with neat supernatant produced with this clone (the stock of this antibody is purified from supernatant).
Western blot - Anti-FOXP3 antibody [mAbcam 450] (ab450)

Datasheet

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Mu L et al. The IDH1 Mutation-Induced Oncometabolite, 2-Hydroxyglutarate, May Affect DNA Methylation and Expression of PD-L1 in Gliomas. Front Mol Neurosci 11:82 (2018). Read more (PubMed: 29643764) »
Jiang H et al. Abnormal acetylation of FOXP3 regulated by SIRT-1 induces Treg functional deficiency in patients with abdominal aortic aneurysms. Atherosclerosis 271:182-192 (2018). IP . Read more (PubMed: 29524861) »

See all 15 Publications for this product

ab450 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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レビューを送る質問を送る

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (tonsil)

Specification

tonsil

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution

Permeabilization

Blocking step

5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

投稿 Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunoprecipitation

Sample

Human Cell lysate - whole cell (Human FOXP3 transfected COS cells)

Specification

Human FOXP3 transfected COS cells

Immuno-precipitation step

Protein G

Abcam user community

Verified customer

投稿 Jan 26 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-FOXP3 抗体 [mAbcam 450] (ab450)

製品の概要

製品の特性

関連製品

アプリケーション

ターゲット情報

画像

データシートおよび資料

プロトコール

参考文献

This product has been referenced in:

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [mAbcam 450]

Immunoprecipitation abreview for Anti-FOXP3 antibody [mAbcam 450]