Anti-FOXP3 抗体 [BLR034F] - BSA free (ab243890)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR034F] to FOXP3 - BSA free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
製品の概要
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製品名
Anti-FOXP3 antibody [BLR034F] - BSA free
FOXP3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [BLR034F] to FOXP3 - BSA free -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC, IHC-P, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human FOXP3 aa 275-325. The exact sequence is proprietary. NP_054728.2 and Gene ID 50943.
Database link: Q9BZS1 -
特記事項
This product is sold under License from Bethyl Laboratories, Inc.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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特記事項(精製)
Recombinant antibody was purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
BLR034F -
アイソタイプ
IgG -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243890の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000.
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ICC |
1/100 - 1/500.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
Use at an assay dependent concentration.
Use 6µl/mg lysate. |
特記事項 |
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WB
1/1000. |
ICC
1/100 - 1/500. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. Use 6µl/mg lysate. |
ターゲット情報
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機能
Probable transcription factor. Plays a critical role in the control of immune response. -
関連疾患
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. -
配列類似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
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別名
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
画像
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for FOXP3 using ab243890 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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All lanes : Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : MJ whole cell lysate
Lane 4 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 5 : RPMI-8226 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 30 secondsDetection: Chemiluminesence
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All lanes : Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution
Lane 1 : MJ whole cell lysate at 20 %
Lane 2 : Control IgG in MJ whole cell lysate
Exposure time: 30 seconds -
Formalin-fixed, paraffin-embedded MJ cells labeling FOXP3 using ab243890 in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab243890 は 1 報の論文で使用されています。
- Tao CJ et al. High expression of WTAP is related to poor prognosis in nasopharyngeal carcinoma. Neoplasma 70:229-239 (2023). PubMed: 36964720