Anti-FOXP3 抗体 (ab4728)
Key features and details
- Rabbit polyclonal to FOXP3
- Suitable for: IHC-Fr, WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXP3 antibody
FOXP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXP3 -
由来種
Rabbit -
特異性
This antibody recognises a single band representing human FOXP3 in a HEK293 lysate overexpressing human FOXP3. -
アプリケーション
適用あり: IHC-Fr, WB, IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
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ポジティブ･コントロール
- This antibody gave a positive signal in Hek293 lysate over expressing FoxP3 and Human tonsil. IHC-P: FFPE Human tonsil tissue sections.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use a concentration of 1 µg/ml. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能
Probable transcription factor. Plays a critical role in the control of immune response. -
関連疾患
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. -
配列類似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 50943 Human
- Omim: 300292 Human
- SwissProt: Q9BZS1 Human
- Unigene: 247700 Human
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別名
- AIID antibody
- DIETER antibody
- Forkhead box P3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab4728)IHC image of FOXP3 staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab4728, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Frozen sections) - Anti-FOXP3 antibody (ab4728)IHC image of FOXP3 staining in a section of formalin-fixed frozen normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was then incubated with ab4728, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Anti-FOXP3 antibody (ab4728)Western blot using ab4728 at 1/500.
Lane 1: HEK 293 lysate with ab4728
Lane 2: HEK 293 lysate over expressing human FOXP3 with ab4728
Lane 3: HEK 293 lysate over expressing mouse FOXP3 with ab4728
Lane 4: HEK 293 lysate with secondary antibody control ab6721.Secondary antibody: Goat polyclonal to Rabbit IgG (HRP) ab6721 1/5000.
Exposure time: 30 sec.
Expected molecular weight: 47kD -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody (ab4728)IHC image of FOXP3 staining in human tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4728, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
プロトコール
データシートおよび資料
参考文献 (9)
ab4728 は 9 報の論文で使用されています。
- Chen J et al. Contribution of regulatory T cells to immune tolerance and association of microRNA-210 and Foxp3 in preeclampsia. Mol Med Rep 19:1150-1158 (2019). PubMed: 30569125
- Huang L et al. CD8+ T cells with high TGF-ß1 expression cause lymph node fibrosis following HIV infection. Mol Med Rep 18:77-86 (2018). PubMed: 29749506
- Isayeva T et al. The protective effect of p16(INK4a) in oral cavity carcinomas: p16(Ink4A) dampens tumor invasion-integrated analysis of expression and kinomics pathways. Mod Pathol 28:631-53 (2015). PubMed: 25523612
- Hvarness T et al. Phenotypic characterisation of immune cell infiltrates in testicular germ cell neoplasia. J Reprod Immunol 100:135-45 (2013). IHC-P ; Human . PubMed: 24290033
- Yu TW et al. Using whole-exome sequencing to identify inherited causes of autism. Neuron 77:259-73 (2013). PubMed: 23352163
- Lee S et al. Prognostic impact of FOXP3 expression in triple-negative breast cancer. Acta Oncol 52:73-81 (2013). PubMed: 23075422
- Popmihajlov Z & Smith KA Negative feedback regulation of T cells via interleukin-2 and FOXP3 reciprocity. PLoS ONE 3:e1581 (2008). Flow Cyt ; Human . PubMed: 18324310
- Oh U et al. Reduced Foxp3 Protein Expression Is Associated with Inflammatory Disease during Human T Lymphotropic Virus Type 1 Infection. J Infect Dis 193:1557-66 (2006). PubMed: 16652285
- Grant C et al. Foxp3 represses retroviral transcription by targeting both NF-kappaB and CREB pathways. PLoS Pathog 2:e33 (2006). PubMed: 16652169
