Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spleen)
Permeabilization
Yes - antigen retrieval (citrus buffer, pH 6.0)
Specification
spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in 5 % goat serum
Dilution
1/50
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Rhodamine conjugated goat to mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Rhodamine
Host species: Goat
Clonality: Polyclonal
Conjugation: Rhodamine
Additional data
Additional Notes
The antibody did not work on the rat spleen, but positively stained cells were observed in human eye sections. The positive staining was later confirmed by another FOXP3 antibody (ab215206).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Danhong Zhu
Verified customer
投稿 Aug 14 2019