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  1. Link

    foxp2-antibody-c-terminal-ab1307.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box
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Anti-FOXP2 抗体 - C-terminal (ab1307)

  • Datasheet
Reviews (6)Q&A (8)References (18)

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  • Western blot - Anti-FOXP2 antibody - C-terminal (ab1307)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody - C-terminal (ab1307)
  • Immunohistochemistry (Frozen sections) - Anti-FOXP2 antibody - C-terminal (ab1307)
  • IHC - Wholemount - Anti-FOXP2 antibody - C-terminal (ab1307)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody - C-terminal (ab1307)

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関連製品

  • データシート
  • 文献 (18)
  • プロトコール

製品の概要

  • 製品名

    Anti-FOXP2 antibody - C-terminal
    FOXP2 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to FOXP2 - C-terminal
  • 由来種

    Goat
  • 特異性

    This antibody is expected to recognise all three reported isoforms (NP_055306.1; NP_683696.2; NP_683697.1).
  • アプリケーション

    適用あり: WB, IHC-P, IHC - Wholemount, ICC/IF, IHC-Frmore details
  • 種交差性

    交差種: Mouse, Human, Zebra finch
    交差が予測される動物種: Rat, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human FOXP2 aa 703-715 (C terminal) (Cysteine residue).
    Sequence:

    C-REIEEEPLSEDLE


    Database link: O15409
    (Peptide available as ab22800)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: Whole tissue lysate from human cerebellum. IHC-Fr: e12.5 mouse embryonic spinal cord tissue. IHC-P: Human testis tissue. ICC/IF: Mouse e12.5 thoracic spinal cord cells. IHC-wholemount: Mouse brain tissue.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXP

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human FOXP2 protein (ab131969)

アプリケーション

Our Abpromise guarantee covers the use of ab1307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 79.9 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 16901978
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 23967072
IHC-Fr Use at an assay dependent concentration. PubMed: 20484636

ターゲット情報

  • 機能

    Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
  • 組織特異性

    Isoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.
  • 関連疾患

    Defects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
    Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2).
  • 配列類似性

    Contains 1 C2H2-type zinc finger.
    Contains 1 fork-head DNA-binding domain.
  • 発生段階

    Expressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber.
  • ドメイン

    The leucine-zipper is required for dimerization and transcriptional repression.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession O15409 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 449627 Chimpanzee
    • Entrez Gene: 101150977 Gorilla
    • Entrez Gene: 93986 Human
    • Entrez Gene: 114142 Mouse
    • Entrez Gene: 500037 Rat
    • Entrez Gene: 613237 Rhesus monkey
    • Entrez Gene: 734154 Xenopus laevis
    • Omim: 605317 Human
    • SwissProt: Q8MJA0 Chimpanzee
    • SwissProt: Q8MJ99 Gorilla
    • SwissProt: O15409 Human
    • SwissProt: P58463 Mouse
    • SwissProt: P0CF24 Rat
    • SwissProt: Q8MJ97 Rhesus monkey
    • SwissProt: Q4VYS1 Xenopus laevis
    • Unigene: 282787 Human
    • Unigene: 332919 Mouse
    • Unigene: 225249 Rat
    • Unigene: 54023 Xenopus laevis
    see all
  • 別名

    • CAG repeat protein 44 antibody
    • CAGH44 antibody
    • DKFZp686H1726 antibody
    • Forkhead box P2 antibody
    • Forkhead box protein P2 antibody
    • forkhead/winged-helix transcription factor antibody
    • FOX P2 antibody
    • FOXP2 antibody
    • FOXP2_HUMAN antibody
    • HGNC11222 antibody
    • HGNC11956 antibody
    • SPCH 1 antibody
    • SPCH1 antibody
    • TNRC 10 antibody
    • TNRC10 antibody
    • trinucleotide repeat containing 10 antibody
    • Trinucleotide repeat containing gene 10 protein antibody
    • Trinucleotide repeat-containing gene 10 protein antibody
    see all

画像

  • Western blot - Anti-FOXP2 antibody - C-terminal (ab1307)
    Western blot - Anti-FOXP2 antibody - C-terminal (ab1307)
    Anti-FOXP2 antibody - C-terminal (ab1307) at 2 µg/ml + Human cerebellum whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 79.9 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody - C-terminal (ab1307)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody - C-terminal (ab1307)

    Paraffin-embedded human testis tissue stained for FOXP2 with ab1307 at 5 µg/ml in immunohistochemical analysis. Steamed antigen retrieval with citrate buffer pH 6. AP staining.

  • Immunohistochemistry (Frozen sections) - Anti-FOXP2 antibody - C-terminal (ab1307)
    Immunohistochemistry (Frozen sections) - Anti-FOXP2 antibody - C-terminal (ab1307)Image courtesy of Dr F Clotman by Abreview.

    ab1307 staining FOXP2 in e12.5 mouse embryonic spinal cord tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 10% serum for 30 minutes at 20°C followed by incubation with the primary antibody, at a 1/1000 dilution, for 16 hours at 4°C. The secondary antibody used was a donkey anti-sheep (and goat) IgG conjugated to Alexa Fluor® 594 (red) used at a 1/2000 dilution.

    See Abreview

  • IHC - Wholemount - Anti-FOXP2 antibody - C-terminal (ab1307)
    IHC - Wholemount - Anti-FOXP2 antibody - C-terminal (ab1307)Abreview from Verfied customer

    Mouse brain tissue stained for FOXP2 with ab1307 (1/1000 dilution) in immunohistochemical analysis.

    Brain explant taken from E15 embryo and cultured for 1 month. Fixed for 4 hours in 4% PFA, permeabilised with PBS-TritonX 100 (0,25%). FOXP2 antibody (ab1307) was used at 1/1000 and a monoclonal mouse antibody was used to visualize CaBP. Secondary antibody incubated at RT for 2 hours.
    Image shows and overlay of FoxP2-positive deep cerebellar nuclei neurons (red) and CaBP-positive Purkinje cells (green).

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody - C-terminal (ab1307)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody - C-terminal (ab1307)Francius C et al, PLoS One. 2013 Aug 15;8(8):e70325. doi: 10.1371/journal.pone.0070325. Figure 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Mouse e12.5 thoracic spinal cord stained for FOXP2 (green) using ab1307 (1/2000 dilution) in ICC/IF.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

データシートおよび資料

    • Datasheet
  • 参考文献

    This product has been referenced in:

    • Burkett ZD  et al. FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch. Elife 7:N/A (2018). Read more (PubMed: 29360038) »
    • Fong WL  et al. Differential and Overlapping Pattern of Foxp1 and Foxp2 Expression in the Striatum of Adult Mouse Brain. Neuroscience 388:214-223 (2018). Read more (PubMed: 30031127) »
    See all 18 Publications for this product

    ab1307 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 14 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP2 antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 10 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-FOXP2 antibody - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Xenopus laevis Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - triton X
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 21 2018

    IHC - Wholemount abreview for Anti-FOXP2 antibody

    Average
    Abreviews
    Abreviews
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Brain)
    Specification
    Brain
    Read More

    Ms. Lea Hachigian

    Verified customer

    投稿 Jul 23 2014

    Western blot abreview for Anti-FOXP2 antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Sample
    Mouse Cell lysate - whole cell (Brain)
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 23 2014

    IHC - Wholemount abreview for Anti-FOXP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Brain explant)
    Specification
    Brain explant
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 28 2012

    Question

    Thank you very much for the new antibody and for your collaboration

    Read More

    Abcam community

    Verified customer

    Asked on Jun 06 2012

    Answer

    That's my pleasure.

    If you have any further questions in the future please do not hesitate to contact us again.

    Until then, I wish you all the best with your research.

    Read More

    Abcam Scientific Support

    Answered on Jun 06 2012

    Question

    i’m sorry but the product is still not working for my purpose (vibratome-cut brain slices from adult mice).
    May we have a replacement? If yes, we would like to receive the RABBIT policlonal anti Foxp2 .
    Thank you very much

    Read More

    Abcam community

    Verified customer

    Asked on Jun 05 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with one unit of https://www.abcam.com/ab16046.The order number is *******.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Jun 05 2012

    Question

    I’m using the Anti-FOXP2 antibody goat (ab1307) in immunofluorescence.

    as you suggest, I used an assay dependent dilution.

    I tried a standard protocol with ab1307 at 1:100, 1:1000, 1:2000 (14 hours) in brain slices from adult mice. But it seems that my protocol doesn’t perform correctly with this antibody.

    I checked on the specific references for this product, but the rabbit FoxP2 was used in one of the 3 papers, in another one it is not clearly specified and in the third one (a work made on birds) they had problems with goat anti foxP2 and developed their own antibody in rabbit.

    Could you please send me the protocol you use for your immunofluorescence assays with this specific antibody made in goat?

    Thank you in advance

    Read More

    Abcam community

    Verified customer

    Asked on May 04 2012

    Answer

    Thank you forcontacting us. I am sorry to hear that this antibody is not providing satisfactory results.

    Theuse of this antibody inIHC-Frwasfirst describedby one of our customersand his result andprotocol can be foundon the Abreview: https://www.abcam.com/index.html?datasheet=1307&tab=abreviews&intabreviewid=20555.

    Shouldthesuggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

    Abcam Scientific Support

    Answered on May 04 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-FOXP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (e12.5 mouse embryonic spinal cord)
    Specification
    e12.5 mouse embryonic spinal cord
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Dr. Frederic Clotman

    Verified customer

    投稿 Sep 17 2010

    Question

    Thank you so much for your assistance and the detailed information you sent. Our thoughts were to use the foxp2 antibody for immunohistochemistry on mouse brain tissues. And since unlike Westerns, we wouldn't be able to differentiate the proteins based on size, I am not sure if we could get around the issue of cross-reactivity. Would you keep me posted on any developments or changes with the foxp antibodies? Thanks so very much again.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2005

    Answer

    Thank you for your email. As soon as we receive new information regarding our products, we update the online datasheets. So keep an eye on the datasheets for more information. Also, we're always adding new products, and I do know that we will be adding additional FOXP antibodies to our catalogue in the future. Please let me know if you have any questions in the future!

    Read More

    Abcam Scientific Support

    Answered on Feb 09 2005

    1-10 of 14 Abreviews or Q&A

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