Anti-FOXP1 抗体 (ab93807)
Key features and details
- Rabbit polyclonal to FOXP1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXP1 antibody
FOXP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis, Elephant -
免疫原
Synthetic peptide corresponding to Human FOXP1 aa 627-677.
Sequence:MQAVHPVHVKEEPLDPEEAEGPLSLVTTANHSPDFDHDRDYEDEPVNEDM E
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ポジティブ・コントロール
- HeLa lysate and 293T cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93807の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 75 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 75 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
関連疾患
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
配列類似性
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
ドメイン
The leucine-zipper is required for dimerization and transcriptional repression. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 515903 Cow
- Entrez Gene: 484692 Dog
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Entrez Gene: 297480 Rat
- Entrez Gene: 496386 Xenopus laevis
- Entrez Gene: 779033 Xenopus laevis
- Omim: 605515 Human
see all -
別名
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
画像
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All lanes : Anti-FOXP1 antibody (ab93807) at 0.1 µg/ml
Lane 1 : HeLa lysate at 50 µg
Lane 2 : HeLa lysate at 15 µg
Lane 3 : HeLa lysate at 5 µg
Lane 4 : 293 T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse hybridoma tumor (right) tissues labelling FOXP1 with ab93807 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation of FOXP1 in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93807 at 3µg/mg lysate with goat anti-rabbit light chain antibody conjugated to HRP. Subsequent WB detections was performed using 1µg/ml ab93807.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab93807 は 2 報の論文で使用されています。
- Liu X et al. Knockdown of forkhead box protein P1 alleviates hypoxia reoxygenation injury in H9c2 cells through regulating Pik3ip1/Akt/eNOS and ROS/mPTP pathway. Bioengineered 13:1320-1334 (2022). PubMed: 35000528
- Zhao J et al. A novel long non-coding RNA TTN-AS1/microRNA-589-5p/FOXP1 positive feedback loop increases the proliferation, migration and invasion of pancreatic cancer cell lines. Oncol Lett 22:794 (2021). PubMed: 34584569