Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse brain)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
50 µg
Specification
mouse brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed
Detection
Detection method
Licor
Negative control
FOXP1A heterozygous and homozygous conditional knockout mouse cortex
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 58, 90 kDa Non-specific: 30, 40 kDa
Positive control
WT mouse cortex
Additional data
Additional Notes
samples lysed in RIPA buffer with P8340 protease inhibitor
1: WT mouse cortex
2: heterozygous FOXP1 conditional knockout mouse
3: homozygous FOXP1 conditional knockout mouse
red is FOXP1, green is GAPDH
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Carin Papendorp
Verified customer
投稿 Apr 19 2021