Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) 抗体 - BSA and Azide free (ab248200)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2314(2)] to FOXO1A (phospho T24) + FOXO4/AFX (phospho T28) + FOXO3A (phospho T32) + Foxo6 (phospho T26) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody - BSA and Azide free -
製品の詳細
Rabbit monoclonal [EPR2314(2)] to FOXO1A (phospho T24) + FOXO4/AFX (phospho T28) + FOXO3A (phospho T32) + Foxo6 (phospho T26) - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248200 is the carrier-free version of ab128876.
This antibody was developed as part of a collaboration with Tobais Dansen from the University of Utrecht in the Netherlands.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 2.51 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2314(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248200の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa. |
ターゲット情報
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細胞内局在
FOXO1A: Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. FOXO4/AFX: Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm. FOXO3A: Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Foxo6: Cytoplasmic and Nuclear. When phosphorylated, translocated from nucleus to cytoplasm. High nuclear localization after stimulation with growth factors. -
参照データベース
- Entrez Gene: 100132074 Human
- Entrez Gene: 2308 Human
- Entrez Gene: 2309 Human
- Entrez Gene: 4303 Human
- Omim: 136533 Human
- Omim: 300033 Human
- Omim: 602681 Human
- Omim: 611457 Human
see all
画像
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All lanes : Anti-FOXO1A (pT24)+FOXO3A (pT32)+FOXO4 (pT28)+FOXO6 (pT26) antibody (ab128876) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : 293T transfected with HA-FOXO4/AFX cell lysates
Lane 3 : 293T transfected with HA-FOXO4.A3 (T32, S197 and S262 mutated to A) cell lysates
Predicted band size: 53 kDaThis data was developed using ab128876, the same antibody clone in a different buffer formulation.
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This data was developed using ab128876, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248200 は論文での使用が確認できていません。