Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Loading amount
100000 cells
Specification
Jurkat cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 5% BSA with 0,1% Tween 20 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-goat
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 95 kDa Non-specific: 60, 70, 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 25 2010