Anti-FOXK1/MNF 抗体 (ab18196)
Key features and details
- Rabbit polyclonal to FOXK1/MNF
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXK1/MNF antibody
FOXK1/MNF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXK1/MNF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18196の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000.
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| IP |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
|
WB
1/1000. |
|
IP
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli. -
組織特異性
Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node. -
配列類似性
Contains 1 FHA domain.
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 221937 Human
- Entrez Gene: 17425 Mouse
- SwissProt: P85037 Human
- SwissProt: P42128 Mouse
- Unigene: 487393 Human
- Unigene: 24214 Mouse
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別名
- A630048H08Rik antibody
- AI463295 antibody
- ENSMUSG00000075577 antibody
see all
画像
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Western blot - Anti-FOXK1/MNF antibody (ab18196)This image is courtesy of an anonymous AbreviewAll lanes : Anti-FOXK1/MNF antibody (ab18196) at 1/5000 dilution
Lane 1 : Human H1299 cell lysate
Lane 2 : Mouse embryonic stem cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Western blot - Anti-FOXK1/MNF antibody (ab18196)All lanes : Anti-FOXK1/MNF antibody (ab18196) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Jurkat nuclear extract lysate (ab14844)
Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 7 :SW480 whole cell lysate (ab3957)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-FOXK1/MNF antibody (ab18196)ICC/IF image of ab18196 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18196, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunoprecipitation - Anti-FOXK1/MNF antibody (ab18196)FOXK1/MNF - ChIP Grade was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to FOXK1/MNF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18196.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 96kDa: FOXK1 / MNF.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (20)
ab18196 は 20 報の論文で使用されています。
- Chen W et al. circ-PRKCI targets miR-1294 and miR-186-5p by downregulating FOXK1 expression to suppress glycolysis in hepatocellular carcinoma. Mol Med Rep 23:N/A (2021). PubMed: 33880589
- Feng Y et al. FOXK1 plays an oncogenic role in the progression of hilar cholangiocarcinoma. Mol Med Rep 23:N/A (2021). PubMed: 33300075
- Wu W et al. Transcription factor forkhead box K1 regulates miR-32 expression and enhances cell proliferation in colorectal cancer. Oncol Lett 21:407 (2021). PubMed: 33841568
- Xia YK et al. Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network. Protein Cell 12:557-577 (2021). PubMed: 32683582
- Meng F et al. SNHG1 knockdown upregulates miR-376a and downregulates FOXK1/Snail axis to prevent tumor growth and metastasis in HCC. Mol Ther Oncolytics 21:264-277 (2021). PubMed: 34095464
