Anti-FOXI1 抗体 (ab20454)
Key features and details
- Goat polyclonal to FOXI1
- Suitable for: WB, IHC-P, ChIP, IHC-Fr
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FOXI1 antibody
FOXI1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to FOXI1 -
由来種
Goat -
特異性
This antibody is expected to recognise both reported human isoforms.
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アプリケーション
適用あり: WB, IHC-P, ChIP, IHC-Frmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human FOXI1 aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q12951 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab20454の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
Primary incubation 1 hour at room temperature. |
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| IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 21303954
|
| ChIP |
Use at an assay dependent concentration. PubMed: 20972246
|
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 16932748
An antigen retrieval method using SDS is described in PubMed 16932748 and PubMed 9072183. |
| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. Primary incubation 1 hour at room temperature. |
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IHC-P
Use at an assay dependent concentration. PubMed: 21303954 |
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ChIP
Use at an assay dependent concentration. PubMed: 20972246 |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 16932748 An antigen retrieval method using SDS is described in PubMed 16932748 and PubMed 9072183. |
ターゲット情報
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機能
Transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules. -
組織特異性
Expressed in kidney. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2299 Human
- Omim: 601093 Human
- SwissProt: Q12951 Human
- Unigene: 87236 Human
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別名
- FKH10 antibody
- FKHL10 antibody
- Forkhead (Drosophila) like 10 antibody
see all
画像
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Western blot - Anti-FOXI1 antibody (ab20454)Anti-FOXI1 antibody (ab20454) at 1 µg/ml + Human Testes lysate (35µg protein in RIPA buffer)
Predicted band size: 41 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab20454 は 10 報の論文で使用されています。
- Montoro DT et al. A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature 560:319-324 (2018). PubMed: 30069044
- Plasschaert LW et al. A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte. Nature 560:377-381 (2018). PubMed: 30069046
- Honda K et al. Molecular architecture underlying fluid absorption by the developing inner ear. Elife 6:N/A (2017). IHC ; Mouse . PubMed: 28994389
- Björkgren I et al. Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding. Mol Cell Endocrinol 427:143-54 (2016). PubMed: 26987518
- Guo Q et al. Adam10 Mediates the Choice between Principal Cells and Intercalated Cells in the Kidney. J Am Soc Nephrol N/A:N/A (2014). PubMed: 24904084
- Raft S et al. Ephrin-B2 governs morphogenesis of endolymphatic sac and duct epithelia in the mouse inner ear. Dev Biol 390:51-67 (2014). PubMed: 24583262
- Trepiccione F et al. Evaluation of cellular plasticity in the collecting duct during the recovery of lithium-induced nephrogenic diabetes insipidus. Am J Physiol Renal Physiol N/A:N/A (2013). IHC . PubMed: 23825070
- Murashima A et al. Essential roles of androgen signaling in wolffian duct stabilization and epididymal cell differentiation. Endocrinology 152:1640-51 (2011). IHC-P ; Mouse . PubMed: 21303954
- Lopez E et al. Variants in CFTR untranslated regions are associated with congenital bilateral absence of the vas deferens. J Med Genet 48:152-9 (2011). WB, ChIP ; Human . PubMed: 20972246
- Blomqvist SR et al. Epididymal expression of the forkhead transcription factor Foxi1 is required for male fertility. EMBO J 25:4131-41 (2006). IHC-Fr ; Mouse . PubMed: 16932748
