Anti-FOXG1 抗体 (ab3394)

Reviews (1)Q&A (3)References (2)
Western blot - Anti-FOXG1 antibody (ab3394)

    Key features and details

    • Goat polyclonal to FOXG1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-FOXG1 antibody [EPR18987] (ab196868)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-FOXG1 antibody
      FOXG1 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to FOXG1

    • 由来種

      Goat

    • 特異性

      This antibody will recognise both human FOXG1A and FOXG1B, which are almost completely identical.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Rat

    • 免疫原

      Synthetic peptide corresponding to Human FOXG1 aa 457-469 (C terminal).
      Sequence:

      C-HQNQGSSSNPLIH


      Database link: P55316
      (Peptide available as ab22945)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3394の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 54 kDa).

    A 1 hour primary incubation is recommended for this product. The additonal unknown band at 70kDa was successfully blocked with the immunising peptide.
    特記事項
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 54 kDa).

    A 1 hour primary incubation is recommended for this product. The additonal unknown band at 70kDa was successfully blocked with the immunising peptide.

    ターゲット情報

    • 機能

      Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.

    • 組織特異性

      Expression is restricted to the neurons of the developing telencephalon.

    • 関連疾患

      Defects in FOXG1 are the cause of congenital variant of Rett syndrome (RTTCV) [MIM:613454]. RTTCV is a severe neurodevelopmental disorder with features of classic Rett syndrome but earlier onset in the first months of life. Clinical features include progressive microcephaly, hypotonia, irresponsiveness and irritability in the neonatal period, mental retardation, psychomotor regression and stereotypical movements.

    • 配列類似性

      Contains 1 fork-head DNA-binding domain.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      • 別名

        • BF-1 antibody
        • BF-2 antibody
        • BF1 antibody
        • BF2 antibody
        • Brain factor 1 antibody
        • Brain factor 2 antibody
        • FHKL antibody
        • FKH2 antibody
        • FKHL1 antibody
        • FKHL2 antibody
        • FKHL3 antibody
        • FKHL4 antibody
        • Forkhead box protein G1 antibody
        • Forkhead box protein G1A antibody
        • Forkhead box protein G1B antibody
        • Forkhead box protein G1C antibody
        • Forkhead like 1 antibody
        • Forkhead like 2 antibody
        • Forkhead like 3 antibody
        • Forkhead like 4 antibody
        • Forkhead-related protein FKHL1 antibody
        • Forkhead-related protein FKHL2 antibody
        • Forkhead-related protein FKHL3 antibody
        • FOXG1 antibody
        • FOXG1_HUMAN antibody
        • FOXG1A antibody
        • FOXG1B antibody
        • FOXG1C antibody
        • HBF 1 antibody
        • HBF G2 antibody
        • hBF-2 antibody
        • HBF2 antibody
        • HFK1 antibody
        • HFK2 antibody
        • HFK3 antibody
        • KHL2 antibody
        • Oncogene QIN antibody
        • QIN antibody
        see all

      画像

      • Western blot - Anti-FOXG1 antibody (ab3394)
        Western blot - Anti-FOXG1 antibody (ab3394)
        ab3394 staining (2µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab3394 (0.3 µg/ml) staining of Human Brain lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

      参考文献 (2)

      ab3394 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3394 は 2 報の論文で使用されています。

      • Li JV  et al. Transcriptional repression of AIB1 by FoxG1 leads to apoptosis in breast cancer cells. Mol Endocrinol 27:1113-27 (2013). PubMed: 23660594
      • Regad T  et al. The neural progenitor-specifying activity of FoxG1 is antagonistically regulated by CKI and FGF. Nat Cell Biol 9:531-40 (2007). ICC/IF ; Mouse . PubMed: 17435750

      レビューと Q&A

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      1-4 of 4 Abreviews or Q&A

      Question

      The replacement we received for ab3394, ab18259, also fails to detect FOXG1 in human neural progenitor cell cytochemistry.

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      Answer

      I have issued a free of charge replacement for ab18259, with the order number *****. The replacement is ab3394. To check the status of the order please contact our Customer Service team and reference this number. I suggested in my conversation with your colleague that an alternative to PFA fixation may give a better result this time: ice-cold acetone or methanol, or a 50:50 mixture of the two, for 10-20 minutes, followed by rinsing in buffer and blocking. No permeabilization is necessary. If you have any questions, please contact me.

      Read More

      Question

      No staining of human neuronal progenitor cells.

      Read More
      Answer

      Thanks for contacting us and letting us know about the problems with ab3394. I am sending a free of charge vial of ab18259 on the order ***, which should arrive shortly. Please keep me updated on the results using this replacement, and let me know if you need anything further.

      Read More

      Immunohistochemistry (Frozen sections) abreview for Anti-FOXG1 antibody

       Poor
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (brain)
      Specification
      brain
      Fixative
      Paraformaldehyde
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 11 2005

      Question

      I would like to use BF-1 (Foxg1) antibody on human ES cells-derived neurons (immunocytochemistry). Your web site says the foxg1 AB is ok for Western blot. Any info on ICH? May i have a small aliq. to test this AB on my slides?

      Read More
      Answer

      Thank you very much for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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