Anti-FOXA2 抗体 [7E6] - BSA and Azide free (ab60721)
Key features and details
- Mouse monoclonal [7E6] to FOXA2 - BSA and Azide free
- Suitable for: IP, WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-FOXA2 antibody [7E6] - BSA and Azide free
FOXA2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7E6] to FOXA2 - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: IP, WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Rhesus monkey, Opossum -
免疫原
Recombinant fragment with tag: HLKPEHHYAF NHPFSINNLM SSEQQHHHSH HHHQPHKMDL KAYEQVMHYP GYGSPMPGSL AMGPVTNKTG LDASPLAADT SYYQGVYSRP IMNSS, corresponding to amino acids 363-458 of Human FOXA2
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ポジティブ・コントロール
- HepG2 whole cell lysate (ab7900).
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特記事項
This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
7E6 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab60721の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa). |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
翻訳後修飾
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. -
細胞内局在
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3170 Human
- Entrez Gene: 15376 Mouse
- Entrez Gene: 25099 Rat
- Omim: 600288 Human
- SwissProt: Q9Y261 Human
- SwissProt: P35583 Mouse
- SwissProt: P32182 Rat
- Unigene: 155651 Human
see all -
別名
- Forkhead box A2 antibody
- Forkhead box protein A2 antibody
- FOX A2 antibody
see all
画像
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ab60721 at 10 ug/ml staining FOXA2 in human Hella cells by Immunocytochemistry/ Immunofluorescence.
This image was generated using the ascites version of the product.
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Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) at 1 µg/ml + HepG2 cell lysate at 25 µg
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 42 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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Overlay histogram showing HepG2 cells stained with ab60721 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60721, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
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Immunoprecipitation analysis of FOXA2 transfected lysate using ab60721 and protein A magnetic bead.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab60721 は 20 報の論文で使用されています。
- Liu Y et al. FOXA2-Interacting FOXP2 Prevents Epithelial-Mesenchymal Transition of Breast Cancer Cells by Stimulating E-Cadherin and PHF2 Transcription. Front Oncol 11:605025 (2021). PubMed: 33718155
- Inamori S et al. Modeling early stages of endoderm development in epiblast stem cell aggregates with supply of extracellular matrices. Dev Growth Differ 62:243-259 (2020). PubMed: 32277710
- Kerschner JL et al. A functional genomics approach to investigate the differentiation of iPSCs into lung epithelium at air-liquid interface. J Cell Mol Med 24:9853-9870 (2020). PubMed: 32692488
- Zhong J et al. Plastrum Testudinis Extracts Promote NSC Differentiation into Dopaminergic Neuron by Regulating the Interaction of TET1 and FoxA2. Evid Based Complement Alternat Med 2020:9104135 (2020). PubMed: 32382312
- Hoveizi E & Mohammadi T Differentiation of endometrial stem cells into insulin-producing cells using signaling molecules and zinc oxide nanoparticles, and three-dimensional culture on nanofibrous scaffolds. J Mater Sci Mater Med 30:101 (2019). PubMed: 31473826