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  1. Link

    foxa2-antibody-ab23630.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box FOXA
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Anti-FOXA2 抗体 (ab23630)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (7)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-FOXA2 antibody (ab23630)
  • Western blot - Anti-FOXA2 antibody (ab23630)
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody (ab23630)

Key features and details

  • Rabbit polyclonal to FOXA2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-FOXA2 antibody [EPR4466] (ab108422)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-FOXA2 antibody
    FOXA2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to FOXA2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human FOXA2 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab25739)

  • ポジティブ・コントロール

    • This antibody gave a positive signal in the following lysates: HepG2 Nuclear and Mouse Liver
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • FOXA
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Metabolism
    • Types of disease
    • Obesity

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab23630の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
  • 配列類似性

    Contains 1 fork-head DNA-binding domain.
  • 翻訳後修飾

    Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
  • 細胞内局在

    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus.
  • Target information above from: UniProt accession Q9Y261 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3170 Human
    • Entrez Gene: 15376 Mouse
    • Entrez Gene: 25099 Rat
    • Omim: 600288 Human
    • SwissProt: Q9Y261 Human
    • SwissProt: P35583 Mouse
    • SwissProt: P32182 Rat
    • Unigene: 155651 Human
    • Unigene: 938 Mouse
    • Unigene: 10948 Rat
    see all
  • 別名

    • Forkhead box A2 antibody
    • Forkhead box protein A2 antibody
    • FOX A2 antibody
    • foxa2 antibody
    • FOXA2_HUMAN antibody
    • Hepatic nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3 antibody
    • Hepatocyte nuclear factor 3 beta antibody
    • Hepatocyte nuclear factor 3-beta antibody
    • HNF 3B antibody
    • HNF-3-beta antibody
    • HNF-3B antibody
    • HNF3B antibody
    • MGC19807 antibody
    • TCF 3B antibody
    • TCF-3B antibody
    • TCF3B antibody
    • Transcription factor 3B antibody
    see all

画像

  • Western blot - Anti-FOXA2 antibody (ab23630)
    Western blot - Anti-FOXA2 antibody (ab23630)
    All lanes : Anti-FOXA2 antibody (ab23630) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Hep G2 nuclear extract lysate (ab14660)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?
    Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-FOXA2 antibody (ab23630)
    Western blot - Anti-FOXA2 antibody (ab23630)
    Anti-FOXA2 antibody (ab23630) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody (ab23630)
    Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody (ab23630)
    ICC/IF image of ab23630 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23630, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

プロトコール

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (7)

ab23630 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab23630 は 7 報の論文で使用されています。

  • Dubois V  et al. Endoplasmic reticulum stress actively suppresses hepatic molecular identity in damaged liver. Mol Syst Biol 16:e9156 (2020). PubMed: 32407006
  • Andreu-Cervera A  et al. The Ciliopathy Gene Ftm/Rpgrip1l Controls Mouse Forebrain Patterning via Region-Specific Modulation of Hedgehog/Gli Signaling. J Neurosci 39:2398-2415 (2019). PubMed: 30692221
  • Nett IR  et al. Negative feedback via RSK modulates Erk-dependent progression from naïve pluripotency. EMBO Rep 19:N/A (2018). PubMed: 29895711
  • Liu C  et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells. Stem Cell Reports 11:43-57 (2018). PubMed: 30008328
  • Levardon H  et al. Expansion of Airway Basal Cells and Generation of Polarized Epithelium. Bio Protoc 8:N/A (2018). PubMed: 30009215
  • Du C  et al. MAPK/FoxA2-mediated cigarette smoke-induced squamous metaplasia of bronchial epithelial cells. Int J Chron Obstruct Pulmon Dis 12:3341-3351 (2017). PubMed: 29200841
  • Vethe H  et al. Probing the missing mature ß-cell proteomic landscape in differentiating patient iPSC-derived cells. Sci Rep 7:4780 (2017). PubMed: 28684784

レビューと Q&A

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1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXA2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (embryonic brain)
Specification
embryonic brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0,2% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Read More

Ms. Judit Kuechler

Verified customer

投稿 Jan 11 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXA2 antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Read More

Mr. Antibody Solutions

Verified customer

投稿 Dec 17 2010

Question

Hi



This is fine AS long as we verify the following – if we purchase and test and get a result we will tell them and send them a picture BUT if it doesn’t work do we also get the discount just for having tested it for them too since it is still information for them ( of course using our own positive and negative controls) :



Since you claim that

However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.



Thanks,

Read More

Abcam community

Verified customer

Asked on Dec 14 2012

Answer

DISCOUNT CODE: ABTRIAL-00000
Expiration date: 14. April 2013
Value: £ 234


I am very pleased to hear you would like to accept our offer and test ab23630 in IHC-Fr. This code will give you £ 234 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. It does not matter if the Abreview is positive or negative. Both is valuable data for us and other scientists.


For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the positive or negative Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Abcam Scientific Support

Answered on Dec 14 2012

Question

I don't know how to put my answer direct to Abcam. Could you please pass my answer to them?

We are trying to do Immunofluorescence on cryosection of chicken embryo.

Thank you very much!!

Read More

Abcam community

Verified customer

Asked on Dec 05 2012

Answer

Thank you very much for your interest our AbTrial program.


To our knowledge, ab23630 has not been tested in Cryosections (IHC-Fr). However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

Read More

Abcam Scientific Support

Answered on Dec 05 2012

Question

I would like to try an antibody for a new application not stated in your literature.



Would you consider sample or sale or return?



We are happy to share data with you.



Product is AB23630 – FOXa2 antibody and we wish to use it on Chicken Embryo’s.



Please can you advise?

Read More

Abcam community

Verified customer

Asked on Dec 04 2012

Answer

Thank you for contacting us.

What kind of application do you have in mind? We may be able to give you our AbTrial discount if it is a new application, but we would need to know what kind of application exactly (IHC-P, IHC-Fr e.g.) you want to perform.

We are looking forward to your reply.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Dec 04 2012

Question

Welche Isoformen erkennen Ihre FoxA2 AK?

Read More

Abcam community

Verified customer

Asked on Feb 17 2012

Answer

Vielen Dank für Ihren Anruf.

Wie versprochen habe ich alle unsere FOXA2-Antikörper darauf geprüft, welche Isoformen von FOXA2 sie erkennen. Leider sind alle Immunogene in Regionen lokalisiert, die in beiden Isoformen vorkommen (Details siehe jeweiliges Datenblatt), und daher detektieren die Antikörper immer beide Isoformen. Wir haben also leider keinen Antikörper erhältlich, der zwischen den Isoformen unterscheiden kann.

Es tut mir leid, dass ich keine besseren Nachrichten für Sie habe. Ich hoffe, diese Informationen helfen Ihnen dennoch weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Abcam Scientific Support

Answered on Feb 17 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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