Anti-FOXA1 抗体 (ab5089)
Key features and details
- Goat polyclonal to FOXA1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FOXA1 antibody
FOXA1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to FOXA1 -
由来種
Goat -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig, Xenopus laevis, Zebrafish
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免疫原
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ポジティブ･コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant Human FOXA1 protein
- WB: HepG2 and MCF7 nuclear cell lysates; Mouse liver lysate. ICC: MCF7 and U2OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Ammonium Sulphate Precipitation -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5089の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49.1 kDa).
A 1 hour primary incubation at room temperature is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. |
| ICC |
Use a concentration of 10 µg/ml.
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| 特記事項 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49.1 kDa). A 1 hour primary incubation at room temperature is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. |
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ICC
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis. -
組織特異性
Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3169 Human
- Entrez Gene: 15375 Mouse
- Entrez Gene: 25098 Rat
- Omim: 602294 Human
- SwissProt: P55317 Human
- SwissProt: P35582 Mouse
- SwissProt: P23512 Rat
- Unigene: 163484 Human
see all -
別名
- forkhead box A1 antibody
- Forkhead box protein A1 antibody
- FOX A1 antibody
see all
画像
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Immunocytochemistry - Anti-FOXA1 antibody (ab5089)
Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in MCF7 cells. An Alexa Fluor 488® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Secondary antibody only control: Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488® secondary antibody at 2ug/ml.
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Western blot - Anti-FOXA1 antibody (ab5089)Lane 1 : Anti-FOXA1 antibody (ab5089) at 0.1 µg/ml
Lane 2 : Anti-FOXA1 antibody (ab5089) at 0.3 µg/ml
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) nuclear cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) nuclear cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49.1 kDaWestern blot analysis labeling FOXA1 with ab5089.
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Western blot - Anti-FOXA1 antibody (ab5089)Anti-FOXA1 antibody (ab5089) at 0.1 µg/ml + Mouse liver lysate at 35 µg
Predicted band size: 49.1 kDaWestern blot labeling FOXA1 with ab5089 at 0.1 ug/ml.
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Immunocytochemistry - Anti-FOXA1 antibody (ab5089)Immunofluorescent analysis of paraformaldehyde-fixed, 0.15% TritonX-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling FOXA1 with ab5089 at 10 ug/ml (1h). Image showing nuclear staining in U-2 OS cells. An Alexa Fluor 488® secondary antibody was used at 2 ug/ml. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Secondary antibody only control: Unimmunized goat IgG (10 ug/ml) followed by an Alexa Fluor 488® secondary antibody at 2ug/ml.
データシートおよび資料
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Datasheet download
参考文献 (68)
ab5089 は 68 報の論文で使用されています。
- Saravanan B et al. Ligand dependent gene regulation by transient ERa clustered enhancers. PLoS Genet 16:e1008516 (2020). PubMed: 31905229
- Luo Z & Farnham PJ Genome-wide analysis of HOXC4 and HOXC6 regulated genes and binding sites in prostate cancer cells. PLoS One 15:e0228590 (2020). PubMed: 32012197
- Takaku M et al. Cancer-specific mutation of GATA3 disrupts the transcriptional regulatory network governed by Estrogen Receptor alpha, FOXA1 and GATA3. Nucleic Acids Res 48:4756-4768 (2020). PubMed: 32232341
- Paranjapye A et al. The FOXA1 transcriptional network coordinates key functions of primary human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol N/A:N/A (2020). PubMed: 32432922
- Cato L et al. ARv7 Represses Tumor-Suppressor Genes in Castration-Resistant Prostate Cancer. Cancer Cell 35:401-413.e6 (2019). PubMed: 30773341
レビューと Q&A
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