Anti-FOXA1 抗体 (ab23738)
Key features and details
- Rabbit polyclonal to FOXA1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FOXA1 antibody
FOXA1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FOXA1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-FoFr or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Xenopus laevis
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免疫原
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ポジティブ･コントロール
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23738の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
Use a concentration of 0.7 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 49 kDa).
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| 特記事項 |
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WB
Use a concentration of 0.7 - 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 49 kDa). |
追加情報
Is unsuitable for ICC/IF,IHC-FoFr or IHC-P.
ターゲット情報
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機能
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis. -
組織特異性
Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. -
配列類似性
Contains 1 fork-head DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3169 Human
- Entrez Gene: 15375 Mouse
- Entrez Gene: 25098 Rat
- Omim: 602294 Human
- SwissProt: P55317 Human
- SwissProt: P35582 Mouse
- SwissProt: P23512 Rat
- Unigene: 163484 Human
see all -
別名
- forkhead box A1 antibody
- Forkhead box protein A1 antibody
- FOX A1 antibody
see all
画像
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Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)All lanes : Anti-FOXA1 antibody (ab23738) at 0.7 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : FOXA1 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDaLanes 1 - 4: Merged signal (red and green). Green - ab23738 observed at 49 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab23738 was shown to recognize FOXA1 in wild-type HAP1 cells as signal was lost at the expected MW in FOXA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FOXA1 knockout samples were subjected to SDS-PAGE. Ab23738 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.7 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)Anti-FOXA1 antibody (ab23738) at 1 µg/ml + Human heart tissue lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa (possible cleavage fragment), 31 kDa (possible cross reactivity) -
Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738) -
Western blot - Anti-FOXA1 antibody - ChIP Grade (ab23738)This image is a courtesy of Anonymous AbreviewAll lanes : Anti-FOXA1 antibody (ab23738) at 1/1000 dilution
Lane 1 : Lysate prepared from human LNCaP cells
Lane 2 : Lysate prepared from MCF7 cells
Lane 3 : Lysate prepared from human LNCaP cells with ethanol as vehicle control
Lane 4 : Lysate prepared from human LNCaP cells with 100 nM DHT
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (135)
ab23738 は 135 報の論文で使用されています。
- Li S et al. CRISPR-based knock-in mutagenesis of the pioneer transcription factor FOXA1: optimization of strategies for multi-allelic proteins in cancer cells. FEBS Open Bio 11:1537-1551 (2021). PubMed: 33666335
- Liu Y et al. FOXA2-Interacting FOXP2 Prevents Epithelial-Mesenchymal Transition of Breast Cancer Cells by Stimulating E-Cadherin and PHF2 Transcription. Front Oncol 11:605025 (2021). PubMed: 33718155
- Pan X et al. MicroRNA-1182 and let-7a exert synergistic inhibition on invasion, migration and autophagy of cholangiocarcinoma cells through down-regulation of NUAK1. Cancer Cell Int 21:161 (2021). PubMed: 33750398
- He Y et al. A noncanonical AR addiction drives enzalutamide resistance in prostate cancer. Nat Commun 12:1521 (2021). PubMed: 33750801
- Baca SC et al. Reprogramming of the FOXA1 cistrome in treatment-emergent neuroendocrine prostate cancer. Nat Commun 12:1979 (2021). PubMed: 33785741