Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain: frontal cortex)
Specification
Brain: frontal cortex
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/1500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
An excellent pattern of immunostaining.
I am not familiar with the distribution pattern of this protein, but I see strong granular somatic positivity and also a dendritic field single granule postivity that appears to be associated with fine, positive nerve fibres ( submitted image of adult mouse - red arrowheads).
Both granular patterns appear to be very similar to published results for FMRP distribution in rat cortex however I have not, in a limited reading, observed any nerve fibre positivity in any area of adult rat brain, identically immunostained.
I am not familiar with the distribution pattern of this protein, but I see strong granular somatic positivity and also a dendritic field single granule postivity that appears to be associated with fine, positive nerve fibres ( submitted image of adult mouse - red arrowheads).
Both granular patterns appear to be very similar to published results for FMRP distribution in rat cortex however I have not, in a limited reading, observed any nerve fibre positivity in any area of adult rat brain, identically immunostained.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Sep 01 2010