Anti-FMNL1 抗体 (ab97456)
Key features and details
- Rabbit polyclonal to FMNL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FMNL1 antibody
FMNL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FMNL1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant protein fragment corresponding to a region within amino acids 631 - 873 of Human FMNL1 (NP_005883).
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ポジティブ･コントロール
- 293T, A431, HepG2, MOLT4 and Raji cell lysates. H1299 and HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97456の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 122 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 122 kDa. |
ターゲット情報
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機能
May play a role in the control of cell motility and survival of macrophages (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape. -
組織特異性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the formin homology family.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
ドメイン
The DAD domain regulates activation via by an autoinhibitory interaction with the N-terminus. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments. -
翻訳後修飾
Myristoylation mediates membrane localization and blebbing. -
細胞内局在
Cytoplasm > cell cortex. Cell projection > bleb. Colocalized with F-actin in bleb protrusions and Cytoplasm. Cell membrane. Cytoplasmic vesicle > phagosome. Recruited to actin-rich phagosomes during phagocytosis. Translocates to the plasma membrane upon activation by RAC1. - Information by UniProt
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参照データベース
- Entrez Gene: 752 Human
- Entrez Gene: 57778 Mouse
- Omim: 604656 Human
- SwissProt: O95466 Human
- SwissProt: Q9JL26 Mouse
- Unigene: 100217 Human
- Unigene: 138913 Mouse
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別名
- 8030453N10Rik antibody
- AI553564 antibody
- C17orf1 antibody
see all
画像
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Western blot - Anti-FMNL1 antibody (ab97456)All lanes : Anti-FMNL1 antibody (ab97456) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa
7.5% SDS PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab97456 は 2 報の論文で使用されています。
- Kage F et al. FMNL formins boost lamellipodial force generation. Nat Commun 8:14832 (2017). PubMed: 28327544
- Monzo P et al. Mechanical confinement triggers glioma linear migration dependent on formin FHOD3. Mol Biol Cell 27:1246-61 (2016). Rat . PubMed: 26912794