製品名Fluoxetine hydrochloride, 5-HT reuptake inhibitor
製品の詳細5-HT reuptake inhibitor
生理活性の詳細Potent, selective 5-HT reuptake inhibitor. Antidepressant in vivo. Binds to rat and human 5-HT transporters with Ki values of 2 and 0.9-20 nM, respectively. Shows around 150-900-fold selectivity over 5-HT1A, 5-HT2A, H1, α1, α2-adrenergic and muscarinic receptors.
保存方法Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解性Soluble in water to 10 mM and in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab66047 staining serotonin in SKNSH cells treated with fluoxetine hydrochloride (ab120077), by ICC/IF. Increase of serotonin expression correlates with increased concentration of fluoxetine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120077 (fluoxetine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66047 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 donkey anti-goat polyclonal antibody (ab96931) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120077 は 4 報の論文で使用されています。
- Aller MI et al. Increased Dosage of High-Affinity Kainate Receptor Gene grik4 Alters Synaptic Transmission and Reproduces Autism Spectrum Disorders Features. J Neurosci 35:13619-28 (2015). PubMed: 26446216
- Casal-Dominguez JJ et al. In vivo and in vitro characterization of naltrindole-derived ligands at the ?-opioid receptor. J Psychopharmacol 27:192-202 (2013). PubMed: 23118019
- Greenhill SD & Jones RS Diverse antiepileptic drugs increase the ratio of background synaptic inhibition to excitation and decrease neuronal excitability in neurones of the rat entorhinal cortex in vitro. Neuroscience 167:456-74 (2010). PubMed: 20167261
- Toulme E et al. P2X4 receptors in activated C8-B4 cells of cerebellar microglial origin. J Gen Physiol 135:333-53 (2010). PubMed: 20231374