Key features and details
- Goat polyclonal to Fluorescein (Rhodamine)
- Suitable for: Immunomicroscopy, Flow Cyt
- Reacts with: Species independent
- Conjugation: Rhodamine. Ex: 550nm, Em: 570nm
- Isotype: IgG
製品名Anti-Fluorescein antibody (Rhodamine)
Fluorescein 一次抗体 製品一覧
製品の詳細Goat polyclonal to Fluorescein (Rhodamine)
標識Rhodamine. Ex: 550nm, Em: 570nm
アプリケーション適用あり: Immunomicroscopy, Flow Cytmore details
種交差性交差種: Species independent
Fluorescein conjugated to Goat IgG
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Goat IgG.
保存方法Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
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特記事項（精製）This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Our Abpromise guarantee covers the use of ab7253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
関連性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
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