Anti-Fluorescein 抗体 [4-4-20 (enhanced)] (ab206508)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [4-4-20 (enhanced)] to Fluorescein
- Suitable for: WB, Flow Cyt
- Reacts with: Species independent
製品の概要
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製品名
Anti-Fluorescein antibody [4-4-20 (enhanced)]
Fluorescein 一次抗体 製品一覧 -
製品の詳細
Human monoclonal [4-4-20 (enhanced)] to Fluorescein -
由来種
Human -
特異性
ab206508 is a recombinant monoclonal antibody to Fluorescein which is specific for fluorescein labelled proteins. -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Fluorescein conjugated to Bovine Serum Albumin (BSA). Fluorescein I-bovine serum albumin (FlI-BSA).
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特記事項
This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques. The isotype of this recombinant monoclonal antibody is Human IgG1, kappa.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
4-4-20 (enhanced) -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab206508の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
別名
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab206508 は 1 報の論文で使用されています。
- Jung S et al. Selection for improved protein stability by phage display. J Mol Biol 294:163-80 (1999). PubMed: 10556036