Flotillin 2 / ESA overexpression 293T lysate (whole cell) (ab94259)
製品の概要
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製品名
Flotillin 2 / ESA overexpression 293T lysate (whole cell)
Flotillin 2/ESA ライゼート 製品一覧 -
特記事項
ab94259 is a 293T cell transfected lysate in which Human Flotillin 2 / ESA has been transiently over-expressed using a pCMV-Flotillin 2 / ESA plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究分野
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背景
Function: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. Tissue specificity: In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. Similarity: Belongs to the band 7/mec-2 family. Flotillin subfamily.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
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特記事項 |
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WB
Use at an assay dependent dilution. |
画像
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ab94259 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Flotillin 2/ESA antibody (ab88498) at 1/500 dilution
Lane 1 :Flotillin 2 / ESA overexpression 293T lysate (whole cell) (ab94259)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94259 は論文での使用が確認できていません。