Anti-Flotillin 1 抗体 (ab13493)
Key features and details
- Goat polyclonal to Flotillin 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Flotillin 1 antibody
Flotillin 1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Flotillin 1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog, Pig, Chimpanzee
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免疫原
Synthetic peptide corresponding to Human Flotillin 1 aa 400 to the C-terminus (C terminal). (NP_001305804.1)
Database link: O75955 -
ポジティブ･コントロール
- WB: K562 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13493の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. -
配列類似性
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
細胞内局在
Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 10211 Human
- Omim: 606998 Human
- SwissProt: O75955 Human
- SwissProt: Q767L6 Pig
- Unigene: 179986 Human
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別名
- FLOT 1 antibody
- FLOT1 antibody
- FLOT1_HUMAN antibody
see all
画像
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Western blot - Anti-Flotillin 1 antibody (ab13493)Anti-Flotillin 1 antibody (ab13493) at 0.1 µg/ml + K562 cell lysate (RIPA buffer) at 35 µg
Predicted band size: 49 kDaDetected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (12)
ab13493 は 12 報の論文で使用されています。
- Koh YQ et al. Extracellular Vesicle Proteome of Breast Cancer Patients with and Without Cognitive Impairment Following Anthracycline-based Chemotherapy: An Exploratory Study. Biomark Insights 16:11772719211018204 (2021). PubMed: 34103887
- Hirai K et al. Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy. Sci Transl Med 12:N/A (2020). PubMed: 33298561
- Schmidt F et al. Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal Infections. Cell Rep 32:108017 (2020). PubMed: 32814035
- Mills JT et al. Airway Epithelial Cells Generate Pro-inflammatory Tenascin-C and Small Extracellular Vesicles in Response to TLR3 Stimuli and Rhinovirus Infection. Front Immunol 10:1987 (2019). PubMed: 31497021
- Vaswani K et al. A Method for the Isolation of Exosomes from Human and Bovine Milk. J Nutr Metab 2019:5764740 (2019). PubMed: 31885909
