Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-FLK1 抗体 [Clone 10] (ab24313)

Reviews (1)Q&A (1)References (1)
Western blot - Anti-FLK1 antibody [Clone 10] (ab24313)

    Key features and details

    • Mouse monoclonal [Clone 10] to FLK1
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-FLK1 antibody [Clone 10]

    • 製品の詳細

      Mouse monoclonal [Clone 10] to FLK1

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Saccharomyces cerevisiae

    • 免疫原

      Recombinant fragment corresponding to Saccharomyces cerevisiae FLK1 aa 1-200.

    • ポジティブ･コントロール

      • Mav108 cells expressing recombinant FLK1.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab24313の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).
    特記事項
    WB
    Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).

    ターゲット情報

    • 関連性

      Yeast TUP1 Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type specific genes regulated by the alpha2 and a1/alpha2 repressors. Ssn6 Tupl interacts with class I histone deacetylases required for repression, and recruitment of yeast Tuplp Ssn6p repressor is associated with localised decreases in histone acetylation.

    • 細胞内局在

      Nuclear

    • 参照データベース

      • 別名

        • FLK1 antibody
        • Flocculation suppressor protein antibody
        • Repressor AER2 antibody
        • SFL2 antibody
        • Tup1p antibody
        • UMR7 antibody
        see all

      画像

      • Western blot - Anti-FLK1 antibody [Clone 10] (ab24313)
        Western blot - Anti-FLK1 antibody [Clone 10] (ab24313)Image is courtesy of an anonymous AbReview.
        All lanes : Anti-FLK1 antibody [Clone 10] (ab24313)

        All lanes : Saccharomyces cerevisiae whole cell lysates

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Sheep anti-Mouse polyclonal HRP conjugate at 100 %

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 78 kDa
        Observed band size: 90 kDa why is the actual band size different from the predicted?


        Exposure time: 1 minute

        See Abreview

      参考文献 (1)

      ab24313 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab24313 は 1 報の論文で使用されています。

      • Mei B  et al. The regulatory mechanism and biological significance of the Snail-miR590-VEGFR-NRP1 axis in the angiogenesis, growth and metastasis of gastric cancer. Cell Death Dis 11:241 (2020). PubMed: 32303680

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-TUP1 antibody [Clone 10]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Saccharomyces cerevisiae Cell lysate - whole cell (whole cell)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      15 µg
      Specification
      whole cell
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 30 2015

      Question

      Why is the detected band size 35kDa but the predicted band size 78kDa? Some journals also mentinoed that the detected band size is around 90kDa.

      Read More
      Answer

      Thank you for your enquiry. The cell line used was transfected to express a fragment of the TUP1 protein, which was the antigen used to develop the antibody. This protein fragment has a molecular weight of approximately 35 kDa. Therefore, the 35kDa band was detected in WB. Unfortunately, we have not tested the product using full length protein and therefore, it is possible that the higher band size you observed with yuo r samples could be the full length protein.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com