Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファーPreservative: 0.02% Sodium azide
Concentration information loading...
特記事項（精製）FLIP Antibody is affinity chromatography purified via peptide column.
Our Abpromise guarantee covers the use of ab8421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 55 kDa.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
機能Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
組織特異性Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
配列類似性Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
ドメインThe caspase domain lacks the active sites residues involved in catalysis.
翻訳後修飾Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
- Information by UniProt
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
ab8421 は 14 報の論文で使用されています。
- Dang Q et al. The ?-secretase inhibitor GSI-I interacts synergistically with the proteasome inhibitor bortezomib to induce ALK+ anaplastic large cell lymphoma cell apoptosis. Cell Signal 59:76-84 (2019). PubMed: 30878517
- Hata T et al. GABA, ?-Aminobutyric Acid, Protects Against Severe Liver Injury. J Surg Res 236:172-183 (2019). PubMed: 30694753
- Zong H et al. miR-125b suppresses cellular proliferation by targeting c-FLIP in gallbladder carcinoma. Oncol Lett 18:6822-6828 (2019). PubMed: 31788125
- Zhang C et al. Identification and validation of microRNAs and their targets expressed in osteosarcoma. Oncol Lett 18:5628-5636 (2019). PubMed: 31656545
- Padmanabhan C et al. cFLIP critically modulates apoptotic resistance in epithelial-to-mesenchymal transition. Oncotarget 8:101072-101086 (2017). PubMed: 29254146