Anti-FLIP 抗体 (ab6144)
Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FLIP antibody
FLIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FLIP -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to amino acids 447-464 of Human FLIP.
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ポジティブ･コントロール
- WB: HeLa, Jurkat, and K562 cells. ICC/IF: Jurkat and K562 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
DEAE-Chromatography -
特記事項（精製）
Purified IgG prepared by ion exchange chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6144の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB | (1) |
1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 10 µg/ml. |
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WB
1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ターゲット情報
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機能
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. -
組織特異性
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. -
配列類似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
ドメイン
The caspase domain lacks the active sites residues involved in catalysis. -
翻訳後修飾
Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. - Information by UniProt
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参照データベース
- Entrez Gene: 8837 Human
- Omim: 603599 Human
- SwissProt: O15519 Human
- Unigene: 390736 Human
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別名
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
see all
画像
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Western blot - Anti-FLIP antibody (ab6144)All lanes : Anti-FLIP antibody (ab6144) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : K562 whole cell lysate
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab6144)ab6144 at 10µg/ml staining FLIP in Jurkat cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab6144)Ab6144 at 5μg/ml staining FLIP in K562 cells by ICC/IF (Immunocytochemistry/Immunofluorescence).
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Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab6144)Immunofluorescence of FLIP in Jurkat cells using ab6144 at 10 ug/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab6144 は 7 報の論文で使用されています。
- Tili E et al. microRNA 155 up regulation in the CNS is strongly correlated to Down's syndrome dementia. Ann Diagn Pathol 34:103-109 (2018). PubMed: 29661714
- Zhao S et al. hnRNP K plays a protective role in TNF-a-induced apoptosis in podocytes. Biosci Rep 38:N/A (2018). WB . PubMed: 29724888
- You C et al. ß-catenin decreases acquired TRAIL resistance in non-small-cell lung cancer cells by regulating the redistribution of death receptors. Int J Oncol 53:2258-2268 (2018). PubMed: 30132512
- Al-Lamki RS et al. Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis. Cell Death Dis 7:e2287 (2016). PubMed: 27362805
- Siu PM et al. Response of XIAP, ARC, and FLIP apoptotic suppressors to 8 wk of treadmill running in rat heart and skeletal muscle. J Appl Physiol 99:204-9 (2005). WB ; Rat . PubMed: 15774698
- Siu PM et al. Aging sustains the hypertrophy-associated elevation of apoptotic suppressor X-linked inhibitor of apoptosis protein (XIAP) in skeletal muscle during unloading. J Gerontol A Biol Sci Med Sci 60:976-83 (2005). PubMed: 16127099
- Siu PM et al. Apoptotic responses to hindlimb suspension in gastrocnemius muscles from young adult and aged rats. Am J Physiol Regul Integr Comp Physiol 289:R1015-26 (2005). PubMed: 15919734
