Anti-FLIP 抗体 (ab6144)
Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FLIP antibody
FLIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FLIP -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide, corresponding to amino acids 447-464 of Human FLIP.
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ポジティブ･コントロール
- WB: HeLa, Jurkat, and K562 cells. ICC/IF: Jurkat and K562 cells
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
DEAE-Chromatography -
特記事項（精製）
Purified IgG prepared by ion exchange chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab6144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ターゲット情報
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機能
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. -
組織特異性
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. -
配列類似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
ドメイン
The caspase domain lacks the active sites residues involved in catalysis. -
翻訳後修飾
Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12. - Information by UniProt
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参照データベース
- Entrez Gene: 8837 Human
- Entrez Gene: 12633 Mouse
- Entrez Gene: 117279 Rat
- Omim: 603599 Human
- SwissProt: O15519 Human
- SwissProt: O35732 Mouse
- Unigene: 390736 Human
- Unigene: 336848 Mouse
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別名
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
see all
画像
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Western blot - Anti-FLIP antibody (ab6144)All lanes : Anti-FLIP antibody (ab6144) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : K562 whole cell lysate
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab6144)ab6144 at 10µg/ml staining FLIP in Jurkat cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab6144)Ab6144 at 5μg/ml staining FLIP in K562 cells by ICC/IF (Immunocytochemistry/Immunofluorescence).
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Immunocytochemistry/ Immunofluorescence - Anti-FLIP antibody (ab6144)Immunofluorescence of FLIP in Jurkat cells using ab6144 at 10 ug/ml.
プロトコール
データシートおよび資料
参考文献 (7)
ab6144 は 7 報の論文で使用されています。
- Tili E et al. microRNA 155 up regulation in the CNS is strongly correlated to Down's syndrome dementia. Ann Diagn Pathol 34:103-109 (2018). PubMed: 29661714
- Zhao S et al. hnRNP K plays a protective role in TNF-a-induced apoptosis in podocytes. Biosci Rep 38:N/A (2018). WB . PubMed: 29724888
- You C et al. ß-catenin decreases acquired TRAIL resistance in non-small-cell lung cancer cells by regulating the redistribution of death receptors. Int J Oncol 53:2258-2268 (2018). PubMed: 30132512
- Al-Lamki RS et al. Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis. Cell Death Dis 7:e2287 (2016). PubMed: 27362805
- Siu PM et al. Response of XIAP, ARC, and FLIP apoptotic suppressors to 8 wk of treadmill running in rat heart and skeletal muscle. J Appl Physiol 99:204-9 (2005). WB ; Rat . PubMed: 15774698
- Siu PM et al. Aging sustains the hypertrophy-associated elevation of apoptotic suppressor X-linked inhibitor of apoptosis protein (XIAP) in skeletal muscle during unloading. J Gerontol A Biol Sci Med Sci 60:976-83 (2005). PubMed: 16127099
- Siu PM et al. Apoptotic responses to hindlimb suspension in gastrocnemius muscles from young adult and aged rats. Am J Physiol Regul Integr Comp Physiol 289:R1015-26 (2005). PubMed: 15919734
