Key features and details
- Rabbit polyclonal to FLIP
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.02% Sodium azide
Concentration information loading...
特記事項（精製）Purified IgG prepared by ion exchange chromatography.
Our Abpromise guarantee covers the use of ab6144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
機能Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
組織特異性Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
配列類似性Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
ドメインThe caspase domain lacks the active sites residues involved in catalysis.
翻訳後修飾Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
- Information by UniProt
- c FLIP antibody
- c FLIPL antibody
- c FLIPR antibody
All lanes : Anti-FLIP antibody (ab6144) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : K562 whole cell lysate
Predicted band size: 55 kDa
Observed band size: 55 kDa
ab6144 at 10µg/ml staining FLIP in Jurkat cells by ICC/IF
Ab6144 at 5μg/ml staining FLIP in K562 cells by ICC/IF (Immunocytochemistry/Immunofluorescence).
Immunofluorescence of FLIP in Jurkat cells using ab6144 at 10 ug/ml.
ab6144 は 7 報の論文で使用されています。
- Tili E et al. microRNA 155 up regulation in the CNS is strongly correlated to Down's syndrome dementia. Ann Diagn Pathol 34:103-109 (2018). PubMed: 29661714
- Zhao S et al. hnRNP K plays a protective role in TNF-a-induced apoptosis in podocytes. Biosci Rep 38:N/A (2018). WB . PubMed: 29724888
- You C et al. ß-catenin decreases acquired TRAIL resistance in non-small-cell lung cancer cells by regulating the redistribution of death receptors. Int J Oncol 53:2258-2268 (2018). PubMed: 30132512
- Al-Lamki RS et al. Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis. Cell Death Dis 7:e2287 (2016). PubMed: 27362805
- Siu PM et al. Response of XIAP, ARC, and FLIP apoptotic suppressors to 8 wk of treadmill running in rat heart and skeletal muscle. J Appl Physiol 99:204-9 (2005). WB ; Rat . PubMed: 15774698
- Siu PM et al. Aging sustains the hypertrophy-associated elevation of apoptotic suppressor X-linked inhibitor of apoptosis protein (XIAP) in skeletal muscle during unloading. J Gerontol A Biol Sci Med Sci 60:976-83 (2005). PubMed: 16127099
- Siu PM et al. Apoptotic responses to hindlimb suspension in gastrocnemius muscles from young adult and aged rats. Am J Physiol Regul Integr Comp Physiol 289:R1015-26 (2005). PubMed: 15919734