Anti-Flightless I 抗体 [EPR4202(2)] (ab109015)
![Western blot - Anti-Flightless I antibody [EPR4202(2)] (ab109015)](https://www.abcam.co.jp/ps/products/109/ab109015/Images/ab109015-121587-anti-flightless-i-antibody-epr42022-western-blot.jpg "Western blot - Anti-Flightless I antibody [EPR4202(2)] (ab109015)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4202(2)] to Flightless I
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Flightless I antibody [EPR4202(2)]
Flightless I 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4202(2)] to Flightless I -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human heart, HeLa, A431, and A549 lysates; Human muscle tissue; HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4202(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109015の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 145 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
追加情報
Is unsuitable for Flow Cyt or IP.
ターゲット情報
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機能
May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration. -
組織特異性
Strongest expression in skeletal muscle with high expression also in the heart and lung. -
関連疾患
Deletion of the FLII gene may be a cause of Smith-Magenis syndrome (SMS) [MIM:182290]. It is a contiguous gene deletion syndrome involving developmental abnormalities and mental retardation. The spectrum of clinical findings includes short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances, and behavioral problems. -
配列類似性
Contains 5 gelsolin-like repeats.
Contains 15 LRR (leucine-rich) repeats. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Colocalizes to actin-rich structures in blastocysts and, together with HRAS1, RHOA and CDC42, in migrating fibroblasts. Localizes to centrosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 2314 Human
- Omim: 600362 Human
- SwissProt: Q13045 Human
- Unigene: 513984 Human
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別名
- Fli 1 antibody
- FLI antibody
- Fli1 antibody
see all
画像
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Western blot - Anti-Flightless I antibody [EPR4202(2)] (ab109015)All lanes : Anti-Flightless I antibody [EPR4202(2)] (ab109015) at 1/1000 dilution
Lane 1 : Humam heart lysate
Lane 2 : HeLa lysate
Lane 3 : A431 lysate
Lane 4 : A549 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 145 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] (ab109015)](https://www.abcam.co.jp/ps/products/109/ab109015/Images/ab109015-121586-anti-flightless-i-antibody-epr42022-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flightless I antibody [EPR4202(2)] (ab109015)Immunohistochemical analysis of Flightless 1 expression in paraffin-embedded Human muscle tissue using ab109015 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] (ab109015)](https://www.abcam.co.jp/ps/products/109/ab109015/Images/ab109015-121585-anti-flightless-i-antibody-epr42022-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Flightless I antibody [EPR4202(2)] (ab109015)Immunofluorescent staining of Flightless I in HeLa cells using ab109015 at 1/100 dilution. -
![Anti-Flightless I antibody [EPR4202(2)] (ab109015)](https://www.abcam.co.jp/ps/products/109/ab109015/Images/ab109015-4-benefits-of-recombinant-antibodies.png)
Anti-Flightless I antibody [EPR4202(2)] (ab109015)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab109015 は論文での使用が確認できていません。
