Anti-Flavivirus E-glycoprotein 抗体 [HF8-4/C6-6/A9] (ab214334)
Key features and details
- Mouse monoclonal [HF8-4/C6-6/A9] to Flavivirus E-glycoprotein
- Suitable for: Sandwich ELISA, WB, ICC/IF
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
- Isotype: IgG1
製品の概要
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製品名
Anti-Flavivirus E-glycoprotein antibody [HF8-4/C6-6/A9]
Flavivirus E-glycoprotein 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HF8-4/C6-6/A9] to Flavivirus E-glycoprotein -
由来種
Mouse -
特異性
This monoclonal antibody reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV. -
アプリケーション
適用あり: Sandwich ELISA, WB, ICC/IFmore details -
種交差性
交差種: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus -
免疫原
Recombinant full length protein corresponding to Dengue Virus 1 Flavivirus E-glycoprotein aa 281-775.
Database link: P27909 -
ポジティブ・コントロール
- WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells. Sandwich ELISA: DENV1, DENV2 (NGC), DENV2 (16681) DENV3, DENV4, JEV
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Proclin 150
Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640 -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
HF8-4/C6-6/A9 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab214334の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The Flaviviridae are a family of viruses that are primarily spread through arthropod vectors (mainly ticks and mosquitoes). Flaviviridae have monopartite, linear, single-stranded RNA genomes of positive polarity, 9.6- to 12.3-kilobase in length with enveloped and spherical virus particles, about 40-60 nm in diameter. -
参照データベース
- SwissProt: P27909 Dengue virus 1
- SwissProt: P29990 Dengue virus 2
- SwissProt: P29991 Dengue virus 2
- SwissProt: Q6YMS4 Dengue virus 3
- SwissProt: Q2YHF0 Dengue virus 4
- SwissProt: P14403 Japanese encephalitis virus
- SwissProt: P06935 West Nile virus
- SwissProt: Q32ZE1 Zika virus
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別名
- Flavivirus envelope glycoprotein antibody
画像
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Immunofluorescent analysis of DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214334 or an antibody control.
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Immunofluorescent analysis of DENV1, DENV2 infected and uninfected cells labeling Flavivirus E-glycoprotein using ab214334 or an antibody control.
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All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-4/C6-6/A9] (ab214334)
Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV3 (H89) infected C6/36 cell lysate
Lane 4 : DENV4 (H241) infected C6/36 cell lysate
Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : YFV (17D) infected C6/36 cell lysate
Lane 8 : WNV (E101) infected C6/36 cell lysate
Lane 9 : C6/36 uninfected control cell lysate -
Sandwich ELISA analysis of Flavivirus E-glycoprotein expression in DENV1, DENV2 (NGC), DENV2 (16681) DENV3, DENV4, JEV and control using ab214334 as capture antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab214334 は論文での使用が確認できていません。