Anti-FLASH 抗体 (ab8420)
Key features and details
- Rabbit polyclonal to FLASH
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FLASH antibody -
製品の詳細
Rabbit polyclonal to FLASH -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide:
SERFQQLMKLFEKSKC
, corresponding to amino acids 1966/1981 of Human FLASH. (Peptide available as ab8455.) -
ポジティブ･コントロール
- HeLa cell lysate An approximate 220 kDa band can be detected.
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特記事項
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2).
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
一次抗体 備考
A novel mammalian CED-4 homologous was recently identified and cloned in mouse (1) and human (2) and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8 (1). There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity (1,2). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8420の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Human FLASH peptide (ab8455).
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Human FLASH peptide (ab8455). |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. Localizes to discrete nuclear foci. - Information by UniProt
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参照データベース
- Entrez Gene: 9994 Human
- Entrez Gene: 26885 Mouse
- Omim: 606880 Human
- SwissProt: Q9UKL3 Human
- SwissProt: Q9WUF3 Mouse
- Unigene: 558218 Human
- Unigene: 22279 Mouse
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別名
- AA387232 antibody
- C8AP2_HUMAN antibody
- CASP 8 associated protein 2 antibody
see all
画像
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Western blot - Anti-FLASH antibody (ab8420)Anti-FLASH antibody (ab8420) at 0.5 µg/ml + HeLa whole cell lysate
Observed band size: 220 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-FLASH antibody (ab8420)ab8420 at 10µg/ml staining FLASH in Hela cells by ICC/IF
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab8420 は 2 報の論文で使用されています。
- Gajdušková P et al. CDK11 is required for transcription of replication-dependent histone genes. Nat Struct Mol Biol 27:500-510 (2020). PubMed: 32367068
- Barcaroli D et al. FLASH is an essential component of Cajal bodies. Proc Natl Acad Sci U S A : (2006). PubMed: 17003126
