Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Penis)
Specification
Human Penis
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100
Other product details
Dilution
1/50
Incubation time
48 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Dako Envision Labelled Polymer
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The secondary antibody I used was part of the DAKO ENVISION System. This system is based on an HRP labelled polymer that is conjugated to secondary antibodies. It can be used with both mouse and rabbit primary antibodies. Secondary antibody as well as the blocking reagent come ready to use so they don't need to be diluted. The blocking reagent for this kit is a peroxidase blocking step. It does not require you to use a protein blocking such as normal serum. You can do an extra normal serum blocking step if you have high background. For antigen retrieval, I incubated slides in citrate buffer and heated them for 20 minutes.
The picture depicts staining of nerve fibers within corporal tissue of the human penis.
I think the staining may be a little too dark. I would suggest a 1:250 or 1:500 dilution in the future or decreasing the incubation time from 48 to 18-24 hours.
The picture depicts staining of nerve fibers within corporal tissue of the human penis.
I think the staining may be a little too dark. I would suggest a 1:250 or 1:500 dilution in the future or decreasing the incubation time from 48 to 18-24 hours.
Abcam response
Thank you for your feedback and suggestions on using this antibody in IHC. We are delighted to hear that the antibody works in thsi application, as it had not previously been tested.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Gwen Lagoda
Verified customer
投稿 Feb 16 2007