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新型コロナウイルス (COVID-19)に伴う当社の対応について

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    fkbp12-antibody-ab2918.pdf

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Immunology Immune Interventions Transplantation
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Anti-FKBP12 抗体 (ab2918)

  • Datasheet
  • SDS
Reviews (22)Q&A (2)References (19)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody (ab2918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)

Key features and details

  • Rabbit polyclonal to FKBP12
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

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関連製品

製品の概要

  • 製品名

    Anti-FKBP12 antibody
    FKBP12 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to FKBP12
  • 由来種

    Rabbit
  • 特異性

    Detects FK506 binding protein 12 kDa (FKBP12).
  • アプリケーション

    適用あり: IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Rabbit, Cow, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human FKBP12 aa 1-13 (N terminal).
    Sequence:

    GVQVETISPGDGR


    (Peptide available as ab4935)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins. FKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Immune Interventions
    • Transplantation

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant human FKBP12 protein (ab85840)
  • Recombinant Protein

    • Recombinant human FKBP12 protein (ab85840)

アプリケーション

Our Abpromise guarantee covers the use of ab2918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • 配列類似性

    Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
    Contains 1 PPIase FKBP-type domain.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession P62942 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2280 Human
    • Entrez Gene: 14225 Mouse
    • Entrez Gene: 25639 Rat
    • Entrez Gene: 379069 Xenopus laevis
    • Omim: 186945 Human
    • SwissProt: P18203 Cow
    • SwissProt: P62942 Human
    • SwissProt: P26883 Mouse
    • SwissProt: P62943 Rabbit
    • SwissProt: Q62658 Rat
    • SwissProt: O42123 Xenopus laevis
    • Unigene: 471933 Human
    • Unigene: 700839 Human
    • Unigene: 278458 Mouse
    • Unigene: 381214 Mouse
    • Unigene: 80611 Rat
    • Unigene: 960 Xenopus laevis
    see all
  • 別名

    • 12 kDa FK506-binding protein antibody
    • 12 kDa FKBP antibody
    • Calstabin 1 antibody
    • FK506 binding protein 1 antibody
    • FK506 binding protein 12 antibody
    • FK506 binding protein 1A (12kD) antibody
    • FK506 binding protein 1A 12kDa antibody
    • FK506 binding protein 1A antibody
    • FK506 binding protein T cell 12 kD antibody
    • FK506 binding protein, T cell, 12 kD antibody
    • FK506 binding protein12 antibody
    • FK506-binding protein 1A antibody
    • FKB1A_HUMAN antibody
    • FKBP 12 antibody
    • FKBP 1A antibody
    • FKBP-12 antibody
    • FKBP-1A antibody
    • FKBP1 antibody
    • FKBP12 antibody
    • FKBP12 Exip3 antibody
    • FKBP12C antibody
    • fkbp1a antibody
    • Immunophilin FKBP12 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
    • PKC12 antibody
    • PKCI2 antibody
    • PPIase antibody
    • PPIase FKBP1A antibody
    • Protein kinase C inhibitor 2 antibody
    • Rotamase antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/50 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody (ab2918)
    Immunocytochemistry/ Immunofluorescence - Anti-FKBP12 antibody (ab2918)
    ICC/IF image of ab2918 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2918, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human heart tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/20 with a rabbit polyclonal antibody recognizing FKBP12 (ab2918) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)This image is courtesy of an Abreview submitted by Miss gwen lagoda

    ab2918 at 1/1000 staining FKBP12 from human penis tissue by IHC-P. The tissue was paraformaldehyde fixed and the slides were incubated in citrate buffer for antigen retrieval and heated for 20 minutes. The tissue was incubated with ab2918 for 24 hours. The secondary antibody used was part of the DAKO ENVISON System. The picture depicts staining of nerve fibers within corporal tissue of the human penis.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP12 antibody (ab2918)This image is courtesy of an Abreview submitted by Miss gwen lagoda
    ab2918 at 1/1000 staining mouse heart tissue sections by IHC-P. The tissue was paraformaldehyde fixed, blocked and then a citrate buffer / heat mediated antigen retireival step was performed. The tissue was incubated with the antibody for 24 hours. An HRP conjugated rabbit polyclonal antibody was used as the secondary.

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (19)

    ab2918 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2918 は 19 報の論文で使用されています。

    • Sánchez-Solano A  et al. Regulation of the Ca2+-activated chloride channel Anoctamin-1 (TMEM16A) by Ca2+-induced interaction with FKBP12 and calcineurin. Cell Calcium 89:102211 (2020). PubMed: 32422433
    • Eshima H  et al. A chronic high-fat diet exacerbates contractile dysfunction with impaired intracellular Ca2+ release capacity in the skeletal muscle of aged mice. J Appl Physiol (1985) 128:1153-1162 (2020). PubMed: 32213111
    • Buckley C  et al. FK506 regulates Ca2+ release evoked by inositol 1,4,5-trisphosphate independently of FK-binding protein in endothelial cells. Br J Pharmacol 177:1131-1149 (2020). PubMed: 31705533
    • Asghari P  et al. Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modification. Elife 9:N/A (2020). PubMed: 31916935
    • Pan Z  et al. Atrial fibrillation and electrophysiology in transgenic mice with cardiac-restricted overexpression of FKBP12. Am J Physiol Heart Circ Physiol 316:H371-H379 (2019). PubMed: 30499712
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 24 Abreviews or Q&A

    Question

    Customer kindly contacteds as they ae getting two bands where the band of interest is expected. Have contacted us before and followed protocol tips provided.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Sep 07 2012

    Western blot abreview for Anti-FKBP12 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Prosate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    Prosate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 06 2017

    Immunoprecipitation abreview for Anti-FKBP12 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (Cultured mESC cells)
    Total protein in input
    2000 µg
    Immuno-precipitation step
    Protein A
    Specification
    Cultured mESC cells
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 05 2017

    Western blot abreview for Anti-FKBP12 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese hamster Cell lysate - whole cell (Chinese hamster ovary (CHO) cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))
    Loading amount
    75000 cells
    Specification
    Chinese hamster ovary (CHO) cells
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 17 2017

    Western blot abreview for Anti-FKBP12 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
    Loading amount
    0.5 µg
    Specification
    Rabbit Reticulocyte
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 05 2016

    Immunoprecipitation abreview for Anti-FKBP12 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
    Total protein in input
    1e+006 cells
    Immuno-precipitation step
    Other - Protein G Dynabeads
    Specification
    Canine Kidney Epithelial MDCK cells
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 04 2016

    Immunoprecipitation abreview for Anti-FKBP12 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))
    Total protein in input
    1e+007 cells
    Immuno-precipitation step
    Other - Protein G Dynabeads
    Specification
    Mouse embryonic fibroblasts (MEFs)
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 04 2016

    Immunoprecipitation abreview for Anti-FKBP12 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (A549 (human lung epithelium cells))
    Total protein in input
    1e+007 cells
    Immuno-precipitation step
    Other - Protein G Dynabeads
    Specification
    A549 (human lung epithelium cells)
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 01 2016

    Western blot abreview for Anti-FKBP12 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    500000 cells
    Specification
    Canine Kidney Epithelial MDCK cells
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 27 2016

    Western blot abreview for Anti-FKBP12 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (VERO E6)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
    Loading amount
    60000 cells
    Specification
    VERO E6
    Blocking step
    Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 11 2016

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