FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) 抗体 [MAC387] (ab7429)

Reviews (1)Q&A (3)References (7)
Flow Cytometry - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)

    Key features and details

    • FITC Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
    • Suitable for: Flow Cyt
    • Reacts with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig, Monkey, Baboon, Cynomolgus monkey, Rhesus monkey
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    製品の概要

    • 製品名

      FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
      S100A9 + Calprotectin (S100A8/A9 complex) 一次抗体 製品一覧

    • 製品の詳細

      FITC Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)

    • 由来種

      Mouse

    • 標識

      FITC. Ex: 493nm, Em: 528nm

    • 特異性

      This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig, Monkey, Baboon, Cynomolgus monkey, Rhesus monkey

    • 免疫原

      Human monocytes

    • 特記事項

      Fluorescein isothiocyanate (FITC) isomer 1.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      Preservative: 0.1% Sodium azide
      Constituent: 0.2% BSA
    • Concentration information loading...

    • 精製度

      Protein G purified

    • 特記事項（精製）

      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

    • ポリ/モノ

      モノクローナル

    • クローン名

      MAC387

    • ミエローマ

      NS1

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab7429 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Flow Cyt Use 10µl for 106 cells.

    Membrane permeabilisation is required for this application.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    画像

    • Flow Cytometry - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)
      Flow Cytometry - FITC Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab7429)

      ab7429 staining S100A9 + Calprotectin (FITC) in human peripheral blood granulocyte by Flow Cytometry analysis.

    参考文献 (7)

    ab7429 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7429 は 7 報の論文で使用されています。

    • Monteiro LN  et al. Tumour-associated macrophages: Relation with progression and invasiveness, and assessment of M1/M2 macrophages in canine mammary tumours. Vet J 234:119-125 (2018). IHC-P . PubMed: 29680383
    • Burwitz BJ  et al. Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease. J Leukoc Biol 96:491-501 (2014). PubMed: 24823811
    • Gutierrez M  et al. The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet Immunol Immunopathol 71:321-34 (1999). PubMed: 10587310
    • Brandtzaeg P  et al. The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathology 21:191-6 (1992). PubMed: 1505941
    • Brandtzaeg P  et al. Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen. J Clin Pathol 41:963-70 (1988). PubMed: 3056988
    • Flavell DJ  et al. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J Histochem Cytochem 35:1217-26 (1987). PubMed: 3309045
    • Taraballi F  et al. Biomimetic collagenous scaffold to tune inflammation by targeting macrophages. J Tissue Eng 7:2041731415624667 (0). IHC . PubMed: 26977285

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Question

    This antibody seems to cross react with granulocytes and monocytes as well. How is the specificity for macrophages determined for this antibody?

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    Answer

    Thank you for contacting us.

    This antibody recognized the L1 or Calprotectin molecule which is expressed by granulocytes, monocytes and tissue macrophages. In staining samples by flow cytometry which contains all of these relevant populations, I would expect positive staining in all groups.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Flow Cytometry abreview for Anti-Macrophage antibody [MAC387] (FITC)

     Average
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (macrophages (peritoneal))
    Specification
    macrophages (peritoneal)
    Read More

    Mr. olivier Reynard

    Verified customer

    投稿 Jul 24 2006

    Question

    I am looking for an antibody to identify macrophage from monocyte by western blot. We have your product CD14 (cat#ab14006-50), but it seems positive for both monocyte and macrophage. Is there any other product we could use (for human cells)?

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    Answer

    After a careful search with the sources of our macrophage antibodies I regret to inform you that we have no products in our product range which can identify macrophage form monocyte by Western blot and are cross reactive with human. In our product range we have ab8173 monoclonal antibody specific for rat macrophage. Unfortunately this antibody is not cross reactive with human. My apologies for the inconvenience,

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    Question

    Is it possible to use the above fluorescently labelled antibody for immunohistochemistry on frozen tissue sections?

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    Answer

    This antibody has not yet been tested in frozen sections.

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