Application
Flow Cytometry
Sample
Rat Cell (Lung)
Permeabilization
Yes - Cytofix/Cytoperm BD Cat: 554714
Gating Strategy
Singlet Cells -> Viable Cells (Vioblue) -> Leucocytes (CD45+ APCy7) -> CD172 (PE) Negative cells -> CD3 (Viogreen) positive Cells
Specification
Lung
Preparation
Cell harvesting/tissue preparation method: Bronchoalveolar Lavage
Sample buffer: PBS + 5% FCS
Sample buffer: PBS + 5% FCS
Fixation
Cytofix/Cytoperm BD Cat: 554714
Other product details
Incubation time
20 minute(s) · Diluent: Room Temperature
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Inespecific binding to Eosinophils
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with Rat and we were unsure of what the results would be.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Eder Fredy Mateus
Verified customer
投稿 Aug 17 2017